Daniel:Notebook/ComboLock/Sequencing: Difference between revisions
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[https://docs.google.com/spreadsheets/d/1F2L2NpqYCXYwYPk1WQdJDkj3yZKMJftYfeYUHtiXCL4/pubhtml# Zhang Lab Indexes] | [https://docs.google.com/spreadsheets/d/1F2L2NpqYCXYwYPk1WQdJDkj3yZKMJftYfeYUHtiXCL4/pubhtml# Zhang Lab Indexes] | ||
==07-08-2016== | '''Sequencing Table''' | ||
Expected length of combolock amplicons with total sequencing length for the forward and reverse directions | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#E6B8B7;font-size:12pt;font-weight:bold" align="center" | |||
| width="105" height="32" | Name | |||
| width="65" | Length (bp) | |||
| width="80" | Forward Seq. Length | |||
| width="65" | Reverse Seq. Length | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | LinkerL | |||
| align="center" align="center" valign="bottom" | 18 | |||
| align="center" align="center" valign="bottom" | 18 | |||
| align="center" align="center" valign="bottom" | 148 | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | Primer2 | |||
| align="center" align="center" valign="bottom" | 18 | |||
| align="center" align="center" valign="bottom" | 36 | |||
| align="center" align="center" valign="bottom" | 130 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | BC-C1 | |||
| align="center" align="center" valign="bottom" | 8 | |||
| align="center" align="center" valign="bottom" | 44 | |||
| align="center" align="center" valign="bottom" | 112 | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | Primer4 | |||
| align="center" align="center" valign="bottom" | 18 | |||
| align="center" align="center" valign="bottom" | 62 | |||
| align="center" align="center" valign="bottom" | 104 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | BCLatch | |||
| align="center" align="center" valign="bottom" | 8 | |||
| align="center" align="center" valign="bottom" | 70 | |||
| align="center" align="center" valign="bottom" | 86 | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | UMI | |||
| align="center" align="center" valign="bottom" | 8 | |||
| align="center" align="center" valign="bottom" | 78 | |||
| align="center" align="center" valign="bottom" | 78 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | Primer6 | |||
| align="center" align="center" valign="bottom" | 18 | |||
| align="center" align="center" valign="bottom" | 96 | |||
| align="center" align="center" valign="bottom" | 70 | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | BC-C2 | |||
| align="center" align="center" valign="bottom" | 8 | |||
| align="center" align="center" valign="bottom" | 104 | |||
| align="center" align="center" valign="bottom" | 52 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | Primer12 | |||
| align="center" align="center" valign="bottom" | 18 | |||
| align="center" align="center" valign="bottom" | 122 | |||
| align="center" align="center" valign="bottom" | 44 | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | BCPadlock | |||
| align="center" align="center" valign="bottom" | 8 | |||
| align="center" align="center" valign="bottom" | 130 | |||
| align="center" align="center" valign="bottom" | 26 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | LinkerR | |||
| align="center" align="center" valign="bottom" | 18 | |||
| align="center" align="center" valign="bottom" | 148 | |||
| align="center" align="center" valign="bottom" | 18 | |||
|} | |||
==Master List== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="164" height="30" | Sample Name | |||
| width="96" | Experiment Start Date | |||
| width="127" | Indexes | |||
| width="300" | Index Sequences | |||
| width="150" | Results | |||
|- style="font-size:12pt" | |||
|style="text-decoration:underline;color:#0000FF" height="15" | [[Daniel:Notebook/ComboLock/2016-6-30|DEJ20160630_CLCProbe_Test2]] | |||
|style="color:#0000FF" align="center" | [[Daniel:Notebook/ComboLock/2016-6-23|6/23/16]] | |||
|style="text-decoration:none" align="center" align="center" | 2,3,5,6 | |||
| | CGATGT, TTAGGC, ACAGTG, GCCAAT | |||
| | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
|style="text-decoration:underline;color:#0000FF" height="30" | [[Daniel:Notebook/ComboLock/2016-7-6|DEJ20160706_CL_P01]] | |||
|style="color:#0000FF" align="center" | [[Daniel:Notebook/ComboLock/2016-6-28|6/28/16]] | |||
|style="text-decoration:none" align="center" | 1-7 | |||
| | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC | |||
| | |||
|- style="font-size:12pt" | |||
|style="text-decoration:underline;color:#0000FF" height="15" | [[Daniel:Notebook/ComboLock/2016-7-6|DEJ20160706_CL_C02]] | |||
|style="color:#0000FF" align="center" | [[Daniel:Notebook/ComboLock/2016-6-29|6/29/16]] | |||
|style="text-decoration:none" align="center" | 10-13 | |||
| | TAGCTT, GGCTAC, CTTGTA, CCATGA | |||
| | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
|style="text-decoration:underline;color:#0000FF" height="15" | [[Daniel:Notebook/ComboLock/2016-7-15|DEJ20160715_C03]] | |||
|style="color:#0000FF" align="center" | [[Daniel:Notebook/ComboLock/2016-7-11|7/11/16]] | |||
|style="text-decoration:none" align="center" | 16-20 | |||
| | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC | |||
| | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-7-22|DEJ20160722_CL_C04]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-7-18|7/18/16]] | |||
| align="center" align="center" valign="bottom" | 21,22,23,26,27 | |||
| valign="bottom" | CAACCC,CTCGGA,TCGAAA,TCGTGA,TACGCG | |||
| | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-7-27|DEJ20160727_CL_C05]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-7-25|7/25/16]] | |||
| align="center" valign="bottom" | 9-13 | |||
| valign="bottom" | GATCAG,TAGCTT,GGCTAC,CTTGTA,CCATGA | |||
| | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-22|DEJ20160822_CL_PC01]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-18|8/18/16]] | |||
| align="center" valign="bottom" | 10-13 | |||
| valign="bottom" | TAGCTT,GGCTAC,CTTGTA,CCATGA | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-8-25|8/25/16]] | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-26|DEJ20160826_CL_PC02]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-22|8/22/16]] | |||
| align="center" valign="bottom" | 33,41,42,44 | |||
| valign="bottom" | CGCAAC,GGGAAC,GAAAAG,GCATCC | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-9-12|9/12/16]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-9-6|DEJ20160906_CL_PC03]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-29|8/29/16]] | |||
| align="center" valign="bottom" | 34-36 | |||
| valign="bottom" | AACTGA,ATCGGG,CAAGCA | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-9-27|9/27/16]] | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-10-13|DEJ201610_CL_PCC01]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-10-7|10/07/16]] | |||
| align="center" valign="bottom" | 24,25 | |||
| valign="bottom" | ATTCGC,TACTGC | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-10-20|10/20/16]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-3|DEJ-CL-RCA01-20161103]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-10-31|10/31/16]] | |||
| align="center" valign="bottom" | 30,31 | |||
| valign="bottom" | TTAGTG,CACCAC | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-11-18|11/18/16]], [[Daniel:Notebook/ComboLock/2016-11-21|11/21/16]], [[Daniel:Notebook/ComboLock/2016-12-6|12/06/16]] | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-16|DEJ-CL-CellC01-20161116]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-7|11/7/16]] | |||
| align="center" valign="bottom" | 25 | |||
| valign="bottom" | TACTGC | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-11-21|11/21/16]], [[Daniel:Notebook/ComboLock/2016-12-6|12/06/16]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-1-9|DEJ20160109_CLv2_PC]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-1-6|1/6/17]] | |||
| align="center" valign="bottom" | 20 | |||
| valign="bottom" | CCTGTC | |||
| align="center" | | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-1-26|DEJ20160109_CLv3_RCA]] and [[Daniel:Notebook/ComboLock/2017-1-30|here]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-1-23|1/23/17]] | |||
| align="center" valign="bottom" | 21-23,25-27 | |||
| valign="bottom" | CAACCC,CTCGGA,TCGAAA,TACTGC,TCGTGA,TACGCG | |||
| align="center" | | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-5-3|DEJ20170503-CLPCv3-04]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-4-28|4/28/17]] | |||
| align="center" valign="bottom" | 20,24-27 | |||
| valign="bottom" | CCTGTC,ATTCGC,TACTGC,TCGTGA,TACGCG | |||
| align="center" | [[Daniel:Notebook/ComboLock/2017-5-16|May 16 2017]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-6-10|DEJ20170610-CLPCv3-05]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-6-5|6/5/17]] | |||
| align="center" valign="bottom" | 20,21,25-27,29 | |||
| valign="bottom" | CCTGTC,CAACCC,TACTGC,TCGTGA,TACGCG,ACGAAC | |||
| align="center" | [[Daniel:Notebook/ComboLock/2017-6-26|June 26, 2017]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-6-20|DEJ20170610-CLAbPC-01]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-6-14|6/14/17]] | |||
| align="center" valign="bottom" | 30-35 | |||
| valign="bottom" | TTAGTG,CACCAC,GCAAGG,CGCAAC,AACTGA,ATCGGG | |||
| align="center" | [[Daniel:Notebook/ComboLock/2017-6-28|June 28, 2017]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-7-11|DEJ20170711-MixPC-01]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-7-6|7/6/17]] | |||
| align="center" valign="bottom" | 20-25 | |||
| valign="bottom" | CCTGTC,CAACCC,CTCGGA,TCGAAA,ATTCGC,TACTGC | |||
| align="center" | | |||
|} | |||
==Sequencing Runs 2017== | |||
===Spring 2017=== | |||
====06-23-2017==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" valign="bottom" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="245" | Index Sequences | |||
| width="90" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-6-10|DEJ20170610-CLPCv3-05]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-6-5|6/5/17]] | |||
| align="center" valign="bottom" | 20,21,25-27,29 | |||
| valign="bottom" | CCTGTC,CAACCC,TACTGC,TCGTGA,TACGCG,ACGAAC | |||
| align="center" | [[Daniel:Notebook/ComboLock/2017-6-26|June 26, 2017]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-6-20|DEJ20170610-CLAbPC-01]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-6-14|6/14/17]] | |||
| align="center" valign="bottom" | 30-35 | |||
| valign="bottom" | TTAGTG,CACCAC,GCAAGG,CGCAAC,AACTGA,ATCGGG | |||
| align="center" | [[Daniel:Notebook/ComboLock/2017-6-28|June 28, 2017]] | |||
|} | |||
====05-08-2017==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" valign="bottom" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="245" | Index Sequences | |||
| width="90" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-5-3|DEJ20170503-CLPCv3-04]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2017-4-28|4/28/17]] | |||
| align="center" valign="bottom" | 20,24-27 | |||
| valign="bottom" | CCTGTC,ATTCGC,TACTGC,TCGTGA,TACGCG | |||
| align="center" | [[Daniel:Notebook/ComboLock/2017-5-16|May 16 2017]] | |||
|} | |||
==Sequencing Runs 2016== | |||
===Summer 2016=== | |||
====07-08-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
Line 25: | Line 297: | ||
| height="30" | [[Daniel:Notebook/ComboLock/2016-7-6|DEJ20160706_CL_P01]] | | height="30" | [[Daniel:Notebook/ComboLock/2016-7-6|DEJ20160706_CL_P01]] | ||
| [[Daniel:Notebook/ComboLock/2016-6-28|6/28/16]] | | [[Daniel:Notebook/ComboLock/2016-6-28|6/28/16]] | ||
| align="center" | | | align="center" | 1-7 | ||
| ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC | | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC | ||
Line 31: | Line 303: | ||
| height="15" | [[Daniel:Notebook/ComboLock/2016-7-6|DEJ20160706_CL_C02]] | | height="15" | [[Daniel:Notebook/ComboLock/2016-7-6|DEJ20160706_CL_C02]] | ||
| [[Daniel:Notebook/ComboLock/2016-6-29|6/29/16]] | | [[Daniel:Notebook/ComboLock/2016-6-29|6/29/16]] | ||
| align="center" | | | align="center" | 10-13 | ||
| TAGCTT, GGCTAC, CTTGTA, CCATGA | | TAGCTT, GGCTAC, CTTGTA, CCATGA | ||
|} | |||
====07-15-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="285" | Index Sequences | |||
|- style="font-size:12pt" | |||
| height="15" | [[Daniel:Notebook/ComboLock/2016-6-30|DEJ20160630_CLCProbe_Test2]] | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-6-23|6/23/16]] | |||
| align="center" align="center" | 2,3,5,6 | |||
| | CGATGT, TTAGGC, ACAGTG, GCCAAT | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
| height="30" | [[Daniel:Notebook/ComboLock/2016-7-6|DEJ20160706_CL_P01]] | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-6-28|6/28/16]] | |||
| align="center" | 1-7 | |||
| | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC | |||
|- style="font-size:12pt" | |||
| height="15" | [[Daniel:Notebook/ComboLock/2016-7-6|DEJ20160706_CL_C02]] | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-6-29|6/29/16]] | |||
| align="center" | 10-13 | |||
| | TAGCTT, GGCTAC, CTTGTA, CCATGA | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
| height="30" | [[Daniel:Notebook/ComboLock/2016-7-15|DEJ20160715_C03]] | |||
| align="center" | [[Daniel:Notebook/ComboLock/2016-7-11|7/11/16]] | |||
| align="center" | 16-20 | |||
| | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC | |||
|} | |||
====08-22-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="285" | Index Sequences | |||
| width="160" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-22|DEJ20160822_CL_PC01]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-18|8/18/16]] | |||
| align="center" valign="bottom" | 10-13 | |||
| valign="bottom" | TAGCTT,GGCTAC,CTTGTA,CCATGA | |||
| valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-25|Analysis and Results]] | |||
|} | |||
====08-26-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="285" | Index Sequences | |||
| width="75" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-26|DEJ20160826_CL_PC02]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-22|8/22/16]] | |||
| align="center" valign="bottom" | 33, 40-42 | |||
| valign="bottom" | CGCAAC,TGACGG,GGGAAC,GAAAAG | |||
| [[Daniel:Notebook/ComboLock/2016-9-12|9/12/16]] | |||
|} | |||
===Fall 2016=== | |||
====09-12-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="285" | Index Sequences | |||
| width="75" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-9-6|DEJ20160906_CL_PC03]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-8-29|8/29/16]] | |||
| align="center" valign="bottom" | 34-36 | |||
| valign="bottom" | AACTGA,ATCGGG,CAAGCA | |||
| [[Daniel:Notebook/ComboLock/2016-9-27|9/27/16]] | |||
|} | |||
====10-18-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="285" | Index Sequences | |||
| width="75" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-10-13|DEJ20161013_CL_PCC01]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-10-7|10/7/16]] | |||
| align="center" valign="bottom" | 24,25 | |||
| valign="bottom" | ATTCGC,TACTGC | |||
| [[Daniel:Notebook/ComboLock/2016-10-20|10/20/16]] | |||
|} | |||
====11-10-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="285" | Index Sequences | |||
| width="75" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-3|DEJ-CL-RCA01-20161103]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-10-31|10/31/16]] | |||
| align="center" valign="bottom" | 30,31 | |||
| valign="bottom" | TTAGTG,CACCAC | |||
| valign="center" | [[Daniel:Notebook/ComboLock/2016-11-18|11/18/16]] | |||
|} | |||
====11-17-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="285" | Index Sequences | |||
| width="75" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-16|DEJ-CL-CellC01-20161116]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-7|11/7/16]] | |||
| align="center" valign="bottom" | 25 | |||
| valign="bottom" | TACTGC | |||
| valign="center" |[[Daniel:Notebook/ComboLock/2016-11-21|11/21/16]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-3|DEJ-CL-RCA01-20161103]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-10-31|10/31/16]] | |||
| align="center" valign="bottom" | 30,31 | |||
| valign="bottom" | TTAGTG,CACCAC | |||
| align="center" valign="center" | [[Daniel:Notebook/ComboLock/2016-11-21|11/21/16]] | |||
|} | |||
====12-05-2016==== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center" | |||
| width="203" height="30" | Sample Name | |||
| width="75" | Experiment Start Date | |||
| width="77" | Indexes | |||
| width="285" | Index Sequences | |||
| width="75" | Results | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-16|DEJ-CL-CellC01-20161116]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-7|11/7/16]] | |||
| align="center" valign="bottom" | 25 | |||
| valign="bottom" | TACTGC | |||
| valign="center" |[[Daniel:Notebook/ComboLock/2016-12-6|12/6/16]] | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-11-3|DEJ-CL-RCA01-20161103]] | |||
| align="center" valign="bottom" | [[Daniel:Notebook/ComboLock/2016-10-31|10/31/16]] | |||
| align="center" valign="bottom" | 30,31 | |||
| valign="bottom" | TTAGTG,CACCAC | |||
| align="center" valign="center" | [[Daniel:Notebook/ComboLock/2016-12-6|12/6/16]] | |||
|} | |} |
Latest revision as of 18:29, 11 July 2017
Sequencing Lists[edit]
This page describes the sequencing samples submitted in the ComboLock project. It keeps track of IDs, etc.
Sequencing Table
Expected length of combolock amplicons with total sequencing length for the forward and reverse directions
Name | Length (bp) | Forward Seq. Length | Reverse Seq. Length |
LinkerL | 18 | 18 | 148 |
Primer2 | 18 | 36 | 130 |
BC-C1 | 8 | 44 | 112 |
Primer4 | 18 | 62 | 104 |
BCLatch | 8 | 70 | 86 |
UMI | 8 | 78 | 78 |
Primer6 | 18 | 96 | 70 |
BC-C2 | 8 | 104 | 52 |
Primer12 | 18 | 122 | 44 |
BCPadlock | 8 | 130 | 26 |
LinkerR | 18 | 148 | 18 |
Master List[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20160630_CLCProbe_Test2 | 6/23/16 | 2,3,5,6 | CGATGT, TTAGGC, ACAGTG, GCCAAT | |
DEJ20160706_CL_P01 | 6/28/16 | 1-7 | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC | |
DEJ20160706_CL_C02 | 6/29/16 | 10-13 | TAGCTT, GGCTAC, CTTGTA, CCATGA | |
DEJ20160715_C03 | 7/11/16 | 16-20 | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC | |
DEJ20160722_CL_C04 | 7/18/16 | 21,22,23,26,27 | CAACCC,CTCGGA,TCGAAA,TCGTGA,TACGCG | |
DEJ20160727_CL_C05 | 7/25/16 | 9-13 | GATCAG,TAGCTT,GGCTAC,CTTGTA,CCATGA | |
DEJ20160822_CL_PC01 | 8/18/16 | 10-13 | TAGCTT,GGCTAC,CTTGTA,CCATGA | 8/25/16 |
DEJ20160826_CL_PC02 | 8/22/16 | 33,41,42,44 | CGCAAC,GGGAAC,GAAAAG,GCATCC | 9/12/16 |
DEJ20160906_CL_PC03 | 8/29/16 | 34-36 | AACTGA,ATCGGG,CAAGCA | 9/27/16 |
DEJ201610_CL_PCC01 | 10/07/16 | 24,25 | ATTCGC,TACTGC | 10/20/16 |
DEJ-CL-RCA01-20161103 | 10/31/16 | 30,31 | TTAGTG,CACCAC | 11/18/16, 11/21/16, 12/06/16
|
DEJ-CL-CellC01-20161116 | 11/7/16 | 25 | TACTGC | 11/21/16, 12/06/16 |
DEJ20160109_CLv2_PC | 1/6/17 | 20 | CCTGTC | |
DEJ20160109_CLv3_RCA and here | 1/23/17 | 21-23,25-27 | CAACCC,CTCGGA,TCGAAA,TACTGC,TCGTGA,TACGCG | |
DEJ20170503-CLPCv3-04 | 4/28/17 | 20,24-27 | CCTGTC,ATTCGC,TACTGC,TCGTGA,TACGCG | May 16 2017 |
DEJ20170610-CLPCv3-05 | 6/5/17 | 20,21,25-27,29 | CCTGTC,CAACCC,TACTGC,TCGTGA,TACGCG,ACGAAC | June 26, 2017 |
DEJ20170610-CLAbPC-01 | 6/14/17 | 30-35 | TTAGTG,CACCAC,GCAAGG,CGCAAC,AACTGA,ATCGGG | June 28, 2017 |
DEJ20170711-MixPC-01 | 7/6/17 | 20-25 | CCTGTC,CAACCC,CTCGGA,TCGAAA,ATTCGC,TACTGC |
Sequencing Runs 2017[edit]
Spring 2017[edit]
06-23-2017[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20170610-CLPCv3-05 | 6/5/17 | 20,21,25-27,29 | CCTGTC,CAACCC,TACTGC,TCGTGA,TACGCG,ACGAAC | June 26, 2017 |
DEJ20170610-CLAbPC-01 | 6/14/17 | 30-35 | TTAGTG,CACCAC,GCAAGG,CGCAAC,AACTGA,ATCGGG | June 28, 2017 |
05-08-2017[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20170503-CLPCv3-04 | 4/28/17 | 20,24-27 | CCTGTC,ATTCGC,TACTGC,TCGTGA,TACGCG | May 16 2017 |
Sequencing Runs 2016[edit]
Summer 2016[edit]
07-08-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences |
DEJ20160630_CLCProbe_Test2 | 6/23/16 | 2,3,5,6 | CGATGT, TTAGGC, ACAGTG, GCCAAT |
DEJ20160706_CL_P01 | 6/28/16 | 1-7 | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC |
DEJ20160706_CL_C02 | 6/29/16 | 10-13 | TAGCTT, GGCTAC, CTTGTA, CCATGA |
07-15-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences |
DEJ20160630_CLCProbe_Test2 | 6/23/16 | 2,3,5,6 | CGATGT, TTAGGC, ACAGTG, GCCAAT |
DEJ20160706_CL_P01 | 6/28/16 | 1-7 | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC |
DEJ20160706_CL_C02 | 6/29/16 | 10-13 | TAGCTT, GGCTAC, CTTGTA, CCATGA |
DEJ20160715_C03 | 7/11/16 | 16-20 | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC |
08-22-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20160822_CL_PC01 | 8/18/16 | 10-13 | TAGCTT,GGCTAC,CTTGTA,CCATGA | Analysis and Results |
08-26-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20160826_CL_PC02 | 8/22/16 | 33, 40-42 | CGCAAC,TGACGG,GGGAAC,GAAAAG | 9/12/16 |
Fall 2016[edit]
09-12-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20160906_CL_PC03 | 8/29/16 | 34-36 | AACTGA,ATCGGG,CAAGCA | 9/27/16 |
10-18-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20161013_CL_PCC01 | 10/7/16 | 24,25 | ATTCGC,TACTGC | 10/20/16 |
11-10-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ-CL-RCA01-20161103 | 10/31/16 | 30,31 | TTAGTG,CACCAC | 11/18/16 |
11-17-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ-CL-CellC01-20161116 | 11/7/16 | 25 | TACTGC | 11/21/16 |
DEJ-CL-RCA01-20161103 | 10/31/16 | 30,31 | TTAGTG,CACCAC | 11/21/16
|
12-05-2016[edit]
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ-CL-CellC01-20161116 | 11/7/16 | 25 | TACTGC | 12/6/16 |
DEJ-CL-RCA01-20161103 | 10/31/16 | 30,31 | TTAGTG,CACCAC | 12/6/16
|