Daniel:Notebook/ComboLock/2016-7-27: Difference between revisions
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>Djacobse (Created page with "=Latch 100 and Dextran Test (Started 7-25-2016= Back to Calendar ==Library Prep (from [[Daniel:Notebook...") |
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Revision as of 16:22, 28 July 2016
Latch 100 and Dextran Test (Started 7-25-2016
Library Prep (from yesterday
- Ethanol precipitation (from yesterday)
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Combined 15 uL from each sample
- Store DNA at 4C
Nanodrop Results
Source | ng/uL |
7-26 (Latch100/Dextran) A | 31.0 |
7-26 (Latch100/Dextran) B | 19.3 |
Sample | ng/uL | uL added | final mass (ng) |
7-21 Sample A | 44.5 | 15 | 667.5 |
7-21 Sample B | 13.9 | 15 | 208.5 |