Daniel:Notebook/ComboLock/2016-7-27: Difference between revisions
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=Latch 100 and Dextran Test (Started [[Daniel:Notebook/ComboLock/2016-7-25|7-25-2016]]= | =Latch 100 and Dextran Test (Started [[Daniel:Notebook/ComboLock/2016-7-25|7-25-2016]])= | ||
[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] | ||
==Library Prep (from [[Daniel:Notebook/ComboLock/2016-7-26|yesterday]]== | ==Library Prep (from [[Daniel:Notebook/ComboLock/2016-7-26|yesterday]])== | ||
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Latest revision as of 16:24, 28 July 2016
Latch 100 and Dextran Test (Started 7-25-2016)[edit]
Library Prep (from yesterday)[edit]
- Ethanol precipitation
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Combined 15 uL from each sample
- Store DNA at 4C
Nanodrop Results[edit]
Source | ng/uL |
7-26 (Latch100/Dextran) A | 31.0 |
7-26 (Latch100/Dextran) B | 19.3 |
Sample | ng/uL | uL added | final mass (ng) |
7-26 Sample A | 31 | 15 | 465 |
7-26 Sample B | 19.3 | 15 | 289.5 |