Daniel:Notebook/ComboLock/2016-9-6: Difference between revisions

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>Djacobse
>Djacobse
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<li>Combined 15 uL from each of the two tubes above</li>
<li>Combined 15 uL from each of the two tubes above</li>
<li>Stored at 4C in upcoming libraries box</li>
<li>Stored at 4C in upcoming libraries box</li>
<li>Sample '''DEJ-CL-PC03'''</li>
<ol>
<ol>



Revision as of 16:20, 9 September 2016

X3 Dilution Test (8/29/2016)

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Protocol

  1. Ethanol Precipitation
    1. Pellet by centrifuging for 25 minutes at 12000rpm at 4C
    2. Remove supernatant and add 750 uL chilled 70% EtOH
    3. Centrifuge for 12 minutes at 12000rpm at 4C
    4. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    5. Add 20 uL nfH2O and measure in nanodrop

    Nanodrop Results

    Sample ng/uL
    Sample A 31
    Sample B 19.3

    Sequencing Library

    1. Combined 15 uL from each of the two tubes above
    2. Stored at 4C in upcoming libraries box
    3. Sample DEJ-CL-PC03
      1. Sample ng/uL uL added final mass (ng)
        9-6 Sample A 31 15 465
        9-6 Sample B 19.3 15 289.5