Daniel:Notebook/ComboLock/2016-9-23: Difference between revisions

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<li>16C hold</li>
<li>16C hold</li>
</ol></ol>
</ol></ol>
===qPCR Sample Table===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="background-color:#948A54;font-size:12pt;font-weight:bold" align="center"
| width="69" height="45" | Sample
| width="65" | Molecule Count
| width="65" | Lane
| width="65" | AmpR Index
| width="65" | Sample Vol (uL)
| width="65" | 2X Kapa SYBR qPCR MM
| width="65" | 100 uM AmpF 6.4
| width="65" | 10 uM AmpR6.3-IndXX
| width="65" | H2O
| width="65" | Total Volume (uL)
| width="65" | Master Mix
|- style="background-color:#D9D9D9;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1A
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | A1
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1B
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | B1
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="background-color:#D9D9D9;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1C
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | C1
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1A
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | D1
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#D9D9D9;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1B
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | E1
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1C
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | F1
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#D9D9D9;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | NTC
| align="center" align="center" valign="bottom" | 0
| align="center" valign="bottom" | H1
| align="center" align="center" valign="bottom" | 36
| align="center" align="center" valign="bottom" | 0
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 19.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | NTC
|- style="background-color:#B8CCE4;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1A
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | A3
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1B
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | B3
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="background-color:#B8CCE4;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1C
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | C3
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1A
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | D3
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#B8CCE4;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1B
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | E3
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1C
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | F3
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#B8CCE4;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | NTC
| align="center" align="center" valign="bottom" | 0
| align="center" valign="bottom" | H3
| align="center" align="center" valign="bottom" | 36
| align="center" align="center" valign="bottom" | 0
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 19.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | NTC
|- style="background-color:#E6B8B7;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1A
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | A5
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1B
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | B5
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="background-color:#E6B8B7;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1C
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | C5
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1A
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | D5
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#E6B8B7;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1B
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | E5
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 1C
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | F5
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#E6B8B7;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | NTC
| align="center" align="center" valign="bottom" | 0
| align="center" valign="bottom" | H5
| align="center" align="center" valign="bottom" | 36
| align="center" align="center" valign="bottom" | 0
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 19.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | NTC
|- style="background-color:#D8E4BC;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2A
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | A7
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2B
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | B7
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="background-color:#D8E4BC;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2C
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | C7
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2A
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | D7
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#D8E4BC;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2B
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | E7
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2C
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | F7
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#D8E4BC;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | NTC
| align="center" align="center" valign="bottom" | 0
| align="center" valign="bottom" | H7
| align="center" align="center" valign="bottom" | 36
| align="center" align="center" valign="bottom" | 0
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 19.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | NTC
|- style="background-color:#FCD5B4;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2A
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | A9
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2B
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | B9
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="background-color:#FCD5B4;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2C
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | C9
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2A
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | D9
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#FCD5B4;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2B
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | E9
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2C
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | F9
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#FCD5B4;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | NTC
| align="center" align="center" valign="bottom" | 0
| align="center" valign="bottom" | H9
| align="center" align="center" valign="bottom" | 36
| align="center" align="center" valign="bottom" | 0
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 19.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | NTC
|- style="background-color:#CCC0DA;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2A
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | A12
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2B
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | B12
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="background-color:#CCC0DA;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2C
| align="center" align="center" valign="bottom" | 1.00E+10
| align="center" valign="bottom" | C12
| align="center" align="center" valign="bottom" | 34
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 17.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMX
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2A
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | D12
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#CCC0DA;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2B
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | E12
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | Sample 2C
| align="center" align="center" valign="bottom" | 1.00E+09
| align="center" valign="bottom" | F12
| align="center" align="center" valign="bottom" | 35
| align="center" align="center" valign="bottom" | 2
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.1
| align="center" align="center" valign="bottom" | 1
| align="center" align="center" valign="bottom" | 21.9
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | MMO
|- style="background-color:#CCC0DA;font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | NTC
| align="center" align="center" valign="bottom" | 0
| align="center" valign="bottom" | H12
| align="center" align="center" valign="bottom" | 36
| align="center" align="center" valign="bottom" | 0
| align="center" align="center" valign="bottom" | 25
| align="center" align="center" valign="bottom" | 0.5
| align="center" align="center" valign="bottom" | 5
| align="center" align="center" valign="bottom" | 19.5
| align="center" align="center" valign="bottom" | 50
| align="center" valign="bottom" | NTC
|}

Revision as of 19:15, 23 September 2016

PCR Testing (Started Yesterday

Back to Calendar

Protocol (all)

  1. Circularization
    1. Heat inactivate enzyme by incubating for 2 minutes at 94C
  2. Exonuclease Digestion
    1. Prepare exonuclease I/III mix by mixing 20 uL exonuclease I (20 units/μL) and 4 uL exonuclease III (100 units/μL) in 1:1 ratio
    2. Add 2 μL of exonuclease I/III mix to reaction
    3. Mix the reaction by swirling pipette around the well 5 times
    4. Incubate reaction at 37 ºC for 2 hours
    5. Heat inactivate enzyme by incubating at 94C for 5 minutes


Standard qPCR like I normally do. Control version for the tests after.

  1. qPCR
    1. Follow reaction map below
      • Note:"X"=5 uL primer per reaction; "O" indicates 1 uL primer
      • Note:"TN" is a range of temperatures (gradient) from 50C (Lane 1) to 65C (Lane 12)
        1 2 3 4 5 6 7 8 9 10 11 12
      A X,T1   X,T2   X,T3   X,T4   X,T5     X,T6
      B X,T1   X,T2   X,T3   X,T4   X,T5     X,T6
      C X,T1   X,T2   X,T3   X,T4   X,T5     X,T6
      D O,T1   O,T2   O,T3   O,T4   O,T5     O,T6
      E O,T1   O,T2   O,T3   O,T4   O,T5     O,T6
      F O,T1   O,T2   O,T3   O,T4   O,T5     O,T6
      G NTC   NTC   NTC   NTC   NTC     NTC
      H                        
    2. Set up reaction according to table in next section
    3. Make master mixes according to following table
    4.   Master Mix
      Reagent MMX MMO NTC
      PrimerF (100 uM) 9.05 1.81 3.05
      PrimerR (10 uM) 90.5 18.1 30.5
      KAPA Master Mix 452.5 452.5 152.5
      nfH2O 316.75 396.39 118.95
    5. Add 48 uL correct master mix to each well
    6. qPCR Cycles
      1. 95C 3 min
      2. 95C 3 sec
      3. 55C 30 sec
      4. 72C 20 sec
      5. plate read
      6. goto b x24
      7. 72C 2 min
      8. 16C hold

    Extension Time

    1. qPCR
      1. Set up reaction according to table
      2. Master Mix
        1. 480 uL KAPA SyberFast MM
        2. 336 uL nfH2O
        3. 9.5 uL AmpF 6.4
      3. Add 43 uL Master Mix to each well
      4. qPCR Cycles
        1. 95C 3 min
        2. 95C 3 sec
        3. 55C 30 sec
        4. 72C 20 sec
        5. plate read
        6. goto b x20
        7. 72C 2 min
        8. 16C hold

      qPCR Sample Table

      Sample Molecule Count Lane AmpR Index Sample Vol (uL) 2X Kapa SYBR qPCR MM 100 uM AmpF 6.4 10 uM AmpR6.3-IndXX H2O Total Volume (uL) Master Mix
      Sample 1A 1.00E+10 A1 34 2 25 0.5 5 17.5 50 MMX
      Sample 1B 1.00E+10 B1 34 2 25 0.5 5 17.5 50 MMX
      Sample 1C 1.00E+10 C1 34 2 25 0.5 5 17.5 50 MMX
      Sample 1A 1.00E+09 D1 35 2 25 0.1 1 21.9 50 MMO
      Sample 1B 1.00E+09 E1 35 2 25 0.1 1 21.9 50 MMO
      Sample 1C 1.00E+09 F1 35 2 25 0.1 1 21.9 50 MMO
      NTC 0 H1 36 0 25 0.5 5 19.5 50 NTC
      Sample 1A 1.00E+10 A3 34 2 25 0.5 5 17.5 50 MMX
      Sample 1B 1.00E+10 B3 34 2 25 0.5 5 17.5 50 MMX
      Sample 1C 1.00E+10 C3 34 2 25 0.5 5 17.5 50 MMX
      Sample 1A 1.00E+09 D3 35 2 25 0.1 1 21.9 50 MMO
      Sample 1B 1.00E+09 E3 35 2 25 0.1 1 21.9 50 MMO
      Sample 1C 1.00E+09 F3 35 2 25 0.1 1 21.9 50 MMO
      NTC 0 H3 36 0 25 0.5 5 19.5 50 NTC
      Sample 1A 1.00E+10 A5 34 2 25 0.5 5 17.5 50 MMX
      Sample 1B 1.00E+10 B5 34 2 25 0.5 5 17.5 50 MMX
      Sample 1C 1.00E+10 C5 34 2 25 0.5 5 17.5 50 MMX
      Sample 1A 1.00E+09 D5 35 2 25 0.1 1 21.9 50 MMO
      Sample 1B 1.00E+09 E5 35 2 25 0.1 1 21.9 50 MMO
      Sample 1C 1.00E+09 F5 35 2 25 0.1 1 21.9 50 MMO
      NTC 0 H5 36 0 25 0.5 5 19.5 50 NTC
      Sample 2A 1.00E+10 A7 34 2 25 0.5 5 17.5 50 MMX
      Sample 2B 1.00E+10 B7 34 2 25 0.5 5 17.5 50 MMX
      Sample 2C 1.00E+10 C7 34 2 25 0.5 5 17.5 50 MMX
      Sample 2A 1.00E+09 D7 35 2 25 0.1 1 21.9 50 MMO
      Sample 2B 1.00E+09 E7 35 2 25 0.1 1 21.9 50 MMO
      Sample 2C 1.00E+09 F7 35 2 25 0.1 1 21.9 50 MMO
      NTC 0 H7 36 0 25 0.5 5 19.5 50 NTC
      Sample 2A 1.00E+10 A9 34 2 25 0.5 5 17.5 50 MMX
      Sample 2B 1.00E+10 B9 34 2 25 0.5 5 17.5 50 MMX
      Sample 2C 1.00E+10 C9 34 2 25 0.5 5 17.5 50 MMX
      Sample 2A 1.00E+09 D9 35 2 25 0.1 1 21.9 50 MMO
      Sample 2B 1.00E+09 E9 35 2 25 0.1 1 21.9 50 MMO
      Sample 2C 1.00E+09 F9 35 2 25 0.1 1 21.9 50 MMO
      NTC 0 H9 36 0 25 0.5 5 19.5 50 NTC
      Sample 2A 1.00E+10 A12 34 2 25 0.5 5 17.5 50 MMX
      Sample 2B 1.00E+10 B12 34 2 25 0.5 5 17.5 50 MMX
      Sample 2C 1.00E+10 C12 34 2 25 0.5 5 17.5 50 MMX
      Sample 2A 1.00E+09 D12 35 2 25 0.1 1 21.9 50 MMO
      Sample 2B 1.00E+09 E12 35 2 25 0.1 1 21.9 50 MMO
      Sample 2C 1.00E+09 F12 35 2 25 0.1 1 21.9 50 MMO
      NTC 0 H12 36 0 25 0.5 5 19.5 50 NTC