Daniel:Notebook/ComboLock/2016-10-13: Difference between revisions
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<li>Add 30 (20 for NTC) uL nfH2O to resuspend; pool samples A-C by reusing the water</li> | <li>Add 30 (20 for NTC) uL nfH2O to resuspend; pool samples A-C by reusing the water</li> | ||
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[[Category:ComboLock]] [[Category:20161007]] |
Revision as of 17:57, 13 October 2016
Padlock0301 Test (Started Friday 10-7)
Library Prep
- Size Select Gel
- Centrifuge tubes at 12000 rpm for 1.5 minutes to bring gel to bottom
- Extract supernatant and add to nanosep column
- Centrifuge at 12000 rpm for 1.5 minutes; collect supernatant
- Ethanol Precipitation
- Add 1250 uL 100% EtOH, 50 uL 3M NaOAc, and 1.5 uL glycoblue to each sample
- Incubate at -80C for a while
- Centrifuge for 25 minutes at 12000 rpm at 4C
- Remove supernatant and add 750 uL 70% EtOH
- Centrifuge for 12 minutes at 12000 rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 30 (20 for NTC) uL nfH2O to resuspend; pool samples A-C by reusing the water