Daniel:Notebook/ComboLock/2016-10-20: Difference between revisions

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File:Miseq20161018.idx25.ntc.basequality.png|Index 25 (NTC) Read Quality
File:Miseq20161018.idx25.ntc.basequality.png|Index 25 (NTC) Read Quality
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===Amplification Primers Gel===
This is a gel I ran to test the "cleanliness" of the amplification primers (no qPCR). The recipe for each lane is 2 uL (10 uM) sample, 8 uL TBE, 2 uL 6x dye, 10 uL added to lane.
[[Image:2016-10-20-AmplificationPrimers.png|600px]]

Revision as of 00:04, 21 October 2016

Padlock0301 Test (Started 10/07/2016)

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Sequencing Results

Results from the 10/18 miseq. Recall that Idx24 (ATTCGC) is the experiment index and Idx25 (TACTGC) is the NTC index.

Alignment Results

  Length Idx 24 Hits Idx24 % Reads Idx 25 Reads Idx 25 % Reads
PosConAmpC1_C2 160 0 0.0 0 0.0
PosConAmpC3_C2 160 0 0.0 16 32.7
PosConAmpC1_C2_RevComp 160 0 0.0 0 0.0
PosConAmpC3_C2_RevComp 160 0 0.0 15 30.6
PosConPadlock0301_RevComp 147 133 12.7 0 0.0
PosConPadlock0201_RevComp 152 171 16.3 0 0.0

Base Quality

Amplification Primers Gel

This is a gel I ran to test the "cleanliness" of the amplification primers (no qPCR). The recipe for each lane is 2 uL (10 uM) sample, 8 uL TBE, 2 uL 6x dye, 10 uL added to lane.

File:2016-10-20-AmplificationPrimers.png