Daniel:Notebook/ComboLock/2016-10-20: Difference between revisions
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>Djacobse m (→Base Quality) |
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[[Image:2016-10-20-AmplificationPrimers.png|600px]] | [[Image:2016-10-20-AmplificationPrimers.png|600px]] | ||
[[Category:ComboLock]] [[Category:20161017]] |
Revision as of 18:19, 24 October 2016
Padlock0301 Test (Started 10/07/2016)
Sequencing Results
Results from the 10/18 miseq. Recall that Idx24 (ATTCGC) is the experiment index and Idx25 (TACTGC) is the NTC index.
Alignment Results
Length | Idx 24 Hits | Idx24 % Reads | Idx 25 Reads | Idx 25 % Reads | |
PosConAmpC1_C2 | 160 | 0 | 0.0 | 0 | 0.0 |
PosConAmpC3_C2 | 160 | 0 | 0.0 | 16 | 32.7 |
PosConAmpC1_C2_RevComp | 160 | 0 | 0.0 | 0 | 0.0 |
PosConAmpC3_C2_RevComp | 160 | 0 | 0.0 | 15 | 30.6 |
PosConPadlock0301_RevComp | 147 | 133 | 12.7 | 0 | 0.0 |
PosConPadlock0201_RevComp | 152 | 171 | 16.3 | 0 | 0.0 |
Base Quality
- Miseq20161018.idx24.exp.basequalityhistogram.png
Index 24 (Experiment) Read Quality
- Miseq20161018.idx25.ntc.basequality.png
Index 25 (NTC) Read Quality
Amplification Primers Gel
This is a gel I ran to test the "cleanliness" of the amplification primers (no qPCR). The recipe for each lane is 2 uL (10 uM) sample, 8 uL TBE, 2 uL 6x dye, 10 uL added to lane.