Daniel:Notebook/ComboLock/2016-11-3: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with " Back to Calendar ==Library Prep== <ol start="3"> <li>Ethanol Precipitation</li> <ol type="A" start="2"> <li>Centrifuge for 25 minutes at 12000...")
 
>Djacobse
Line 2: Line 2:
[[Daniel:Notebook/ComboLock|Back to Calendar]]
[[Daniel:Notebook/ComboLock|Back to Calendar]]


==Library Prep==
=Circularization Optimization Testing (Started [[Daniel:Notebook/ComboLock/2016-10-31|Monday]])=
 
==Library Prep (Started [[Daniel:Notebook/ComboLock/2016-11-2|Yesterday]])==


<ol start="3">
<ol start="3">

Revision as of 18:24, 2 November 2016

Back to Calendar

Circularization Optimization Testing (Started Monday)

Library Prep (Started Yesterday)

  1. Ethanol Precipitation
    1. Centrifuge for 25 minutes at 12000 rpm at 4C
    2. Remove supernatant and add 750 uL 70% EtOH
    3. Centrifuge for 12 minutes at 12000 rpm at 4C
    4. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    5. Add 30 (20 for NTC) uL nfH2O to resuspend; pool samples A-C by reusing the water