Daniel:Notebook/ComboLock/2016-11-3: Difference between revisions
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>Djacobse (Created page with " Back to Calendar ==Library Prep== <ol start="3"> <li>Ethanol Precipitation</li> <ol type="A" start="2"> <li>Centrifuge for 25 minutes at 12000...") |
>Djacobse |
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[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] | ||
==Library Prep== | =Circularization Optimization Testing (Started [[Daniel:Notebook/ComboLock/2016-10-31|Monday]])= | ||
==Library Prep (Started [[Daniel:Notebook/ComboLock/2016-11-2|Yesterday]])== | |||
<ol start="3"> | <ol start="3"> |
Revision as of 18:24, 2 November 2016
Circularization Optimization Testing (Started Monday)
Library Prep (Started Yesterday)
- Ethanol Precipitation
- Centrifuge for 25 minutes at 12000 rpm at 4C
- Remove supernatant and add 750 uL 70% EtOH
- Centrifuge for 12 minutes at 12000 rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 30 (20 for NTC) uL nfH2O to resuspend; pool samples A-C by reusing the water