Daniel:Notebook/ComboLock/2017-1-10: Difference between revisions
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>Djacobse (Created page with "=Title= Back to Calendar ng/uL:40.3") |
>Djacobse |
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= | =Version 2 Oligos (Started [[Daniel:Notebook/ComboLock/2017-1-6|1/6/2017]])= | ||
[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] | ||
ng/uL:40.3 | ==Library Prep (Started [[Daniel:Notebook/ComboLock/2017-1-9|Yesterday]])== | ||
<ol start="2"> | |||
<li>Ethanol Precipitation</li> | |||
<ol type="A" start="2"> | |||
<li>Centrifuge for 25 minutes at 12000 rpm at 4C</li> | |||
<li>Remove supernatant and add 750 uL chilled 70% EtOH</li> | |||
<li>Centrifuge for 12 minutes at 12000 rpm at 4C</li> | |||
<li>Remove supernatant and spin in vacuum centrifuge for 10 minutes</li> | |||
<li>Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water</li> | |||
</ol></ol> | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#C4BD97;font-size:12pt;font-weight:bold" align="center" | |||
| width="160" height="34" | Sample | |||
| width="85" | ng/uL | |||
| width="85" | ug in 30 uL | |||
|- style="font-size:12pt" | |||
| height="30" valign="bottom" | Sample Lanes (1A+1B) | |||
| align="center" align="center" | 40.3 | |||
| align="center" align="center" | 1.2 | |||
|} | |||
'''DEJ20170109-CLv2-PC''' | |||
[[Category:ComboLock]] [[Category:20170106]] |
Latest revision as of 21:55, 13 January 2017
Version 2 Oligos (Started 1/6/2017)[edit]
Library Prep (Started Yesterday)[edit]
- Ethanol Precipitation
- Centrifuge for 25 minutes at 12000 rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000 rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water
Sample | ng/uL | ug in 30 uL |
Sample Lanes (1A+1B) | 40.3 | 1.2 |
DEJ20170109-CLv2-PC