Daniel:Notebook/ComboLock/2017-1-10: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
No edit summary
>Djacobse
 
(One intermediate revision by the same user not shown)
Line 22: Line 22:


|- style="font-size:12pt"
|- style="font-size:12pt"
| height="30"  valign="bottom" | Cells RCA Product (2AB,2BB)
| height="30"  valign="bottom" | Sample Lanes (1A+1B)
| align="center" align="center" | 47.8
| align="center" align="center" | 40.3
| align="center" align="center" | 1.4
| align="center" align="center" | 1.2
|}
|}


'''DEJ20170109-CLv2-PC'''
'''DEJ20170109-CLv2-PC'''


ng/uL: 40.3
[[Category:ComboLock]] [[Category:20170106]]

Latest revision as of 21:55, 13 January 2017

Version 2 Oligos (Started 1/6/2017)[edit]

Back to Calendar

Library Prep (Started Yesterday)[edit]

  1. Ethanol Precipitation
    1. Centrifuge for 25 minutes at 12000 rpm at 4C
    2. Remove supernatant and add 750 uL chilled 70% EtOH
    3. Centrifuge for 12 minutes at 12000 rpm at 4C
    4. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    5. Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water
Sample ng/uL ug in 30 uL
Sample Lanes (1A+1B) 40.3 1.2

DEJ20170109-CLv2-PC