Daniel:Notebook/ComboLock/2017-1-30: Difference between revisions
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>Djacobse (Created page with "=RCA Test (Started Monday)= Back to Calendar ==Library Prep (Started [[Daniel:Notebook/ComboLock/2017-1...") |
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<li>Consolidate with samples from [[Daniel:Notebook/ComboLock/2017-1-26|Thursday 1-26]] | <li>Consolidate with samples from [[Daniel:Notebook/ComboLock/2017-1-26|Thursday 1-26]] | ||
</ol></ol> | </ol></ol> | ||
'''DEJ20160126-CLv3-RCA02''' | |||
'''Final Sample Matrix''' | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#8064A2;font-size:12pt;font-weight:bold" align="center" | |||
| width="160" height="30" | Sample | |||
| width="85" | Sample Amt | |||
| width="85" | Pre/Post RCA | |||
| width="85" | AmpR Index | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | Sample 0 (AB) | |||
| align="center" valign="bottom" | 1 pmol | |||
| align="center" valign="bottom" | Pre | |||
| align="center" align="center" valign="bottom" | 21 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |||
| height="15" valign="bottom" | Sample 0X (AB) | |||
| align="center" valign="bottom" | 1 pmol | |||
| align="center" valign="bottom" | Post | |||
| align="center" align="center" valign="bottom" | 25 | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | Sample 2X (AB) | |||
| align="center" valign="bottom" | 10 fmol | |||
| align="center" valign="bottom" | Post | |||
| align="center" align="center" valign="bottom" | 26 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |||
| height="15" valign="bottom" | Sample 4X (AB) | |||
| align="center" valign="bottom" | 100 amol | |||
| align="center" valign="bottom" | Post | |||
| align="center" align="center" valign="bottom" | 27 | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | Sample 6C (AB) | |||
| align="center" valign="bottom" | 1 amol | |||
| align="center" valign="bottom" | Epi | |||
| align="center" align="center" valign="bottom" | 22 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |||
| height="15" valign="bottom" | Sample 6D (AB) | |||
| align="center" valign="bottom" | 1 amol | |||
| align="center" valign="bottom" | Epi 1:10 | |||
| align="center" align="center" valign="bottom" | 23 | |||
|} |
Revision as of 23:25, 30 January 2017
RCA Test (Started Monday)
Library Prep (Started Jan 26)
- Ethanol Precipitation
- Centrifuge for 30 minutes at 12500 rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 15 minutes at 12500 rpm at 4C
- Remove supernatant; dry in vacuum centrifuge for 15 minutes
- Resuspend in 20 uL H2O and consolidate samples
- Measure with Qbit
- Consolidate with samples from Thursday 1-26
DEJ20160126-CLv3-RCA02
Final Sample Matrix
Sample | Sample Amt | Pre/Post RCA | AmpR Index |
Sample 0 (AB) | 1 pmol | Pre | 21 |
Sample 0X (AB) | 1 pmol | Post | 25 |
Sample 2X (AB) | 10 fmol | Post | 26 |
Sample 4X (AB) | 100 amol | Post | 27 |
Sample 6C (AB) | 1 amol | Epi | 22 |
Sample 6D (AB) | 1 amol | Epi 1:10 | 23 |