Daniel:Notebook/ComboLock/2017-2-3: Difference between revisions
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>Djacobse |
>Djacobse |
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(11 intermediate revisions by the same user not shown) | |||
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<li>Rolling Circle Amplification</li> | <li>Rolling Circle Amplification</li> | ||
<ol type="A"> | <ol type="A"> | ||
<li>Prepare 8.2X Master mix according to table below</li> | <li>Prepare 8.2X Master mix according to table below; '''Give A samples LLRC and B Samples LLRC*'''</li> | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
Line 22: | Line 22: | ||
| width="65" | uL Added | | width="65" | uL Added | ||
| width="105" | Master Mix (8.2X) | | width="105" | Master Mix (8.2X) | ||
| width="105" | Master Mix (4.2X) | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
| height="15" valign="bottom" | Template | | height="15" valign="bottom" | Template | ||
| align="center" align="center" valign="bottom" | 5 | | align="center" align="center" valign="bottom" | 5 | ||
| align="center" valign="bottom" | NA | |||
| align="center" valign="bottom" | NA | | align="center" valign="bottom" | NA | ||
Line 31: | Line 33: | ||
| height="15" valign="bottom" | RCA Primer (10 uM) | | height="15" valign="bottom" | RCA Primer (10 uM) | ||
| align="center" align="center" valign="bottom" | 2.5 | | align="center" align="center" valign="bottom" | 2.5 | ||
| align="center" align="center" valign="bottom" | | | align="center" align="center" valign="bottom" | 0 | ||
| align="center" align="center" valign="bottom" | 0 | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
Line 37: | Line 40: | ||
| align="center" align="center" valign="bottom" | 5 | | align="center" align="center" valign="bottom" | 5 | ||
| align="center" align="center" valign="bottom" | 41 | | align="center" align="center" valign="bottom" | 41 | ||
| align="center" align="center" valign="bottom" | 21 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |- style="background-color:#BFBFBF;font-size:12pt" | ||
Line 42: | Line 46: | ||
| align="center" align="center" valign="bottom" | 2 | | align="center" align="center" valign="bottom" | 2 | ||
| align="center" align="center" valign="bottom" | 16.4 | | align="center" align="center" valign="bottom" | 16.4 | ||
| align="center" align="center" valign="bottom" | 8.4 | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
| height="15" valign="bottom" | Phi29 | | height="15" valign="bottom" | Phi29 | ||
| align="center" align="center" valign="bottom" | 1 | | align="center" align="center" valign="bottom" | 1 | ||
| align="center" align="center" valign="bottom" | 0 | |||
| align="center" align="center" valign="bottom" | 0 | | align="center" align="center" valign="bottom" | 0 | ||
Line 52: | Line 58: | ||
| align="center" align="center" valign="bottom" | 0.4 | | align="center" align="center" valign="bottom" | 0.4 | ||
| align="center" align="center" valign="bottom" | 3.28 | | align="center" align="center" valign="bottom" | 3.28 | ||
| align="center" align="center" valign="bottom" | 0 | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
Line 57: | Line 64: | ||
| align="center" align="center" valign="bottom" | 4.1 | | align="center" align="center" valign="bottom" | 4.1 | ||
| align="center" align="center" valign="bottom" | 33.62 | | align="center" align="center" valign="bottom" | 33.62 | ||
| align="center" align="center" valign="bottom" | 18.9 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |- style="background-color:#BFBFBF;font-size:12pt" | ||
| height="15" valign="bottom" | Total | | height="15" valign="bottom" | Total | ||
|style="font-weight:bold" align="center" align="center" valign="bottom" | 20 | |style="font-weight:bold" align="center" align="center" valign="bottom" | 20 | ||
|style="font-weight:bold" align="center" align="center" valign="bottom" | | |style="font-weight:bold" align="center" align="center" valign="bottom" | 114.8 | ||
|style="font-weight:bold" align="center" align="center" valign="bottom" | 58.8 | |||
|} | |||
<li>Add 11.5 uL master mix to each tube</li> | |||
<li>Add sample and 1 uL appropriate enzyme to new reaction tubes</li> | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#B1A0C7;font-size:12pt;font-weight:bold" align="center" | |||
| width="100" height="30" | Sample | |||
| width="65" | 1X (AB) | |||
| width="65" | 2X (AB) | |||
| width="65" | 3X (AB) | |||
| width="65" | 4X (AB) | |||
| width="65" | 1Y (AB) | |||
| width="65" | 4Y (AB) | |||
|- style="font-size:12pt" | |||
| height="15" valign="bottom" | Phi29 Source | |||
| align="center" valign="bottom" | NEB | |||
| align="center" valign="bottom" | NEB | |||
| align="center" valign="bottom" | NEB | |||
| align="center" valign="bottom" | NEB | |||
|style="font-weight:bold" align="center" valign="bottom" | Epicentre | |||
|style="font-weight:bold" align="center" valign="bottom" | Epicentre | |||
|} | |} | ||
<li>Incubate at 37C for 3 hours</li> | <li>Incubate at 37C for 3 hours</li> | ||
<li>Incubate at 65C for 10 minutes;Hold at 10C until next step</li></ol></ol> | <li>Incubate at 65C for 10 minutes;Hold at 10C until next step</li></ol></ol> | ||
Line 75: | Line 106: | ||
<li>qPCR</li> | <li>qPCR</li> | ||
<ol type="A"> | <ol type="A"> | ||
<li>Make | <li>Make 21.2X qPCR master mix according to following recipe</li> | ||
<ol type="a"> | <ol type="a"> | ||
<li> | <li>445.2 uL nfH2O</li> | ||
<li> | <li>530 uL SYBR Fast</li> | ||
<li> | <li>21.2 uL 10 uM AmpF</li> | ||
</ol> | </ol> | ||
<li>Add 47 uL master mix to each well</li> | <li>Add 47 uL master mix to each well</li> | ||
<li>Add 2 uL sample and 1 uL appropriate Reverse Primer (1 uM) according to table in | <li>Add 2 uL sample and 1 uL appropriate Reverse Primer (1 uM) according to table</li> | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#92CDDC;font-size:12pt;font-weight:bold" align="center" | |||
| width="100" height="45" | Sample | |||
| width="130" | Condition | |||
| width="70" | Pre/Post RCA | |||
| width="65" | Lane | |||
| width="65" | AmpR Index | |||
| width="65" | Sample Vol (uL) | |||
| width="65" | 2X Kapa SYBR qPCR MM | |||
| width="65" | 10 uM Forward Primer | |||
| width="65" | 10 uM Reverse Primer | |||
| width="65" | H2O | |||
| width="65" | Total Volume (uL) | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 1A | |||
| align="center" | Sample | |||
| align="center" | Pre | |||
| align="center" | A1 | |||
| align="center" align="center" | 20 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 1B | |||
| align="center" | Sample | |||
| align="center" | Pre | |||
| align="center" | A2 | |||
| align="center" align="center" | 20 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 2A | |||
| align="center" | No Template | |||
| align="center" | Pre | |||
| align="center" | A3 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | Sample 2B | |||
| align="center" valign="bottom" | No Template | |||
| align="center" valign="bottom" | Pre | |||
| align="center" | A4 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 3A | |||
| align="center" | No C Probe | |||
| align="center" | Pre | |||
| align="center" | A5 | |||
| align="center" align="center" | 22 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 3B | |||
| align="center" | No C Probe | |||
| align="center" | Pre | |||
| align="center" | A6 | |||
| align="center" align="center" | 22 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 4A | |||
| align="center" | No Lock Phosphate | |||
| align="center" | Pre | |||
| align="center" | A7 | |||
| align="center" align="center" | 23 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#BFBFBF;font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 4B | |||
| align="center" | No Lock Phosphate | |||
| align="center" valign="bottom" | Pre | |||
| align="center" | A8 | |||
| align="center" align="center" | 23 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 1AX | |||
| align="center" | Sample | |||
| align="center" | Post | |||
| align="center" | H1 | |||
| align="center" align="center" | 24 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#DA9694;font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 1BX | |||
| align="center" | Sample | |||
| align="center" | Post | |||
| align="center" | H2 | |||
| align="center" align="center" | 24 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 2AX | |||
| align="center" | No Template | |||
| align="center" | Post | |||
| align="center" | H3 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#DA9694;font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | Sample 2BX | |||
| align="center" valign="bottom" | No Template | |||
| align="center" valign="bottom" | Post | |||
| align="center" | H4 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 3AX | |||
| align="center" | No C Probe | |||
| align="center" | Post | |||
| align="center" | H5 | |||
| align="center" align="center" | 26 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#DA9694;font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 3BX | |||
| align="center" | No C Probe | |||
| align="center" | Post | |||
| align="center" | H6 | |||
| align="center" align="center" | 26 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 4AX | |||
| align="center" | No Lock Phosphate | |||
| align="center" | Post | |||
| align="center" | H7 | |||
| align="center" align="center" | 27 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#DA9694;font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 4BX | |||
| align="center" | No Lock Phosphate | |||
| align="center" valign="bottom" | Post | |||
| align="center" | H8 | |||
| align="center" align="center" | 27 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 1AY | |||
| align="center" | Sample | |||
| align="center" | Post | |||
| align="center" | D1 | |||
| align="center" align="center" | 28 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#C4D79B;font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 1BY | |||
| align="center" | Sample | |||
| align="center" | Post | |||
| align="center" | D2 | |||
| align="center" align="center" | 28 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" | Sample 4AY | |||
| align="center" | No Lock Phosphate | |||
| align="center" | Post | |||
| align="center" | D3 | |||
| align="center" align="center" | 29 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="background-color:#C4D79B;font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | Sample 4BY | |||
| align="center" valign="bottom" | No Lock Phosphate | |||
| align="center" valign="bottom" | Post | |||
| align="center" | D4 | |||
| align="center" align="center" | 29 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 21 | |||
| align="center" align="center" | 50 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | NTC | |||
| align="center" valign="bottom" | qPCR Primers Only | |||
| align="center" align="center" valign="bottom" | | |||
| align="center" | D5 | |||
| align="center" align="center" | 30 | |||
| align="center" align="center" | 2 | |||
| align="center" align="center" | 25 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 1 | |||
| align="center" align="center" | 23 | |||
| align="center" align="center" | 50 | |||
|} | |||
<li>qPCR Cycles</li> | |||
<ol type="a"> | |||
<li>95C 3 min</li> | |||
<li>95C 3 sec</li> | |||
<li>55C 30 sec</li> | |||
<li>72C 20 sec</li> | |||
<li>plate read</li> | |||
<li>goto b x24</li> | |||
<li>72C 2 min</li> | |||
<li>16C hold</li> | |||
</ol></ol> | |||
<li>TBE Gel</li> | |||
<ol type="A"> | |||
<li>Mix 224 uL TBE, 56 uL 6x loading dye</li> | |||
<li>Aliquot 10 uL per sample/ladder lane onto parafilm</li> | |||
<li>Add 2 uL of sample or ladder to correct drop</li> | |||
<li>Load 10 uL in to well</li> | |||
<li>Run gel for 23 minutes at 230V</li> | |||
<li>Open gel and stain with 3 uL SYBR Gold for 3 minutes</li> | |||
<li>Rinse gel and image in gel doc</li> | |||
</ol></ol> | |||
===Results=== | |||
<gallery perrow=2 heights=300px widths=300px> | |||
File:20170203-qPCR-v2Oligos-Round2.png|qPCR Curve | |||
File:2017-02-03-v2Oligos-Round2-PreRCA.png|Gel Image-PreRCA | |||
File:2017-02-03-v2Oligos-Round2-PostRCA.png|Gel Image-PostRCA | |||
File:2017-02-03-v2Oligos-Round2-Epicentre.png|Gel Image-Epicentre | |||
</gallery> | |||
[[Category:ComboLock]] [[Category:20170202]] |
Latest revision as of 00:18, 4 February 2017
Version 2 Oligos Round 2 (Started yesterday)[edit]
Protocol-Part 4-RCA[edit]
- Exonuclease Digestion
- Mix 15 uL Exo I (20U/uL) and 3 uL ExoIII (100U/uL)
- Add 2 uL to each sample, mix by swirling pipette tip
- Incubate for 1 hour at 37C
- Heat kill by incubating for 5 min at 95C
- Rolling Circle Amplification
- Prepare 8.2X Master mix according to table below; Give A samples LLRC and B Samples LLRC*
- Add 11.5 uL master mix to each tube
- Add sample and 1 uL appropriate enzyme to new reaction tubes
- Incubate at 37C for 3 hours
- Incubate at 65C for 10 minutes;Hold at 10C until next step
Reagent | uL Added | Master Mix (8.2X) | Master Mix (4.2X) |
Template | 5 | NA | NA |
RCA Primer (10 uM) | 2.5 | 0 | 0 |
dNTP (1 mM) | 5 | 41 | 21 |
10X Buffer | 2 | 16.4 | 8.4 |
Phi29 | 1 | 0 | 0 |
BSA (10 mg/mL) | 0.4 | 3.28 | 0 |
nfH2O | 4.1 | 33.62 | 18.9 |
Total | 20 | 114.8 | 58.8 |
Sample | 1X (AB) | 2X (AB) | 3X (AB) | 4X (AB) | 1Y (AB) | 4Y (AB) |
Phi29 Source | NEB | NEB | NEB | NEB | Epicentre | Epicentre |
Protocol Part 5-qPCR and Gel[edit]
- qPCR
- Make 21.2X qPCR master mix according to following recipe
- 445.2 uL nfH2O
- 530 uL SYBR Fast
- 21.2 uL 10 uM AmpF
- Add 47 uL master mix to each well
- Add 2 uL sample and 1 uL appropriate Reverse Primer (1 uM) according to table
- qPCR Cycles
- 95C 3 min
- 95C 3 sec
- 55C 30 sec
- 72C 20 sec
- plate read
- goto b x24
- 72C 2 min
- 16C hold
- TBE Gel
- Mix 224 uL TBE, 56 uL 6x loading dye
- Aliquot 10 uL per sample/ladder lane onto parafilm
- Add 2 uL of sample or ladder to correct drop
- Load 10 uL in to well
- Run gel for 23 minutes at 230V
- Open gel and stain with 3 uL SYBR Gold for 3 minutes
- Rinse gel and image in gel doc
Sample | Condition | Pre/Post RCA | Lane | AmpR Index | Sample Vol (uL) | 2X Kapa SYBR qPCR MM | 10 uM Forward Primer | 10 uM Reverse Primer | H2O | Total Volume (uL) |
Sample 1A | Sample | Pre | A1 | 20 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 1B | Sample | Pre | A2 | 20 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 2A | No Template | Pre | A3 | 21 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 2B | No Template | Pre | A4 | 21 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 3A | No C Probe | Pre | A5 | 22 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 3B | No C Probe | Pre | A6 | 22 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 4A | No Lock Phosphate | Pre | A7 | 23 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 4B | No Lock Phosphate | Pre | A8 | 23 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 1AX | Sample | Post | H1 | 24 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 1BX | Sample | Post | H2 | 24 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 2AX | No Template | Post | H3 | 25 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 2BX | No Template | Post | H4 | 25 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 3AX | No C Probe | Post | H5 | 26 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 3BX | No C Probe | Post | H6 | 26 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 4AX | No Lock Phosphate | Post | H7 | 27 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 4BX | No Lock Phosphate | Post | H8 | 27 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 1AY | Sample | Post | D1 | 28 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 1BY | Sample | Post | D2 | 28 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 4AY | No Lock Phosphate | Post | D3 | 29 | 2 | 25 | 1 | 1 | 21 | 50 |
Sample 4BY | No Lock Phosphate | Post | D4 | 29 | 2 | 25 | 1 | 1 | 21 | 50 |
NTC | qPCR Primers Only | D5 | 30 | 2 | 25 | 1 | 1 | 23 | 50 |
Results[edit]
- 20170203-qPCR-v2Oligos-Round2.png
qPCR Curve
- 2017-02-03-v2Oligos-Round2-PreRCA.png
Gel Image-PreRCA
- 2017-02-03-v2Oligos-Round2-PostRCA.png
Gel Image-PostRCA
- 2017-02-03-v2Oligos-Round2-Epicentre.png
Gel Image-Epicentre