Daniel:Notebook/ComboLock/2017-2-10: Difference between revisions
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>Djacobse (Created page with "=Beads Binding Test (Started Yesterday)= Back to Calendar ==Protocol-Part 2-qPCR== <ol start="4"> <li>q...") |
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==Plate Layouts== | |||
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[[Image:20170209-Plate6-10.png|600px]] |
Revision as of 00:57, 10 February 2017
Beads Binding Test (Started Yesterday)
Protocol-Part 2-qPCR
- qPCR
- Make qPCR master mixes according to following recipes
- Add 48 uL master mix to each well
- Add 2 uL sample according to table in following section
- qPCR Cycles
- 95C 3 min
- 95C 3 sec
- 55C 30 sec
- 72C 15 sec
- plate read
- goto b x24
- 72C 2 min
- 16C hold