Daniel:Notebook/ComboLock/2017-3-25: Difference between revisions
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<li>Stain with 2 uL SYBR gold for 3 minutes</li> | <li>Stain with 2 uL SYBR gold for 3 minutes</li> | ||
<li>Rinse and image in gel doc</li> | <li>Rinse and image in gel doc</li> | ||
</ol> | </ol></ol> | ||
==RCA Protocol== | ==RCA Protocol== |
Revision as of 22:55, 25 March 2017
RCA Test
This experiment is to test the effectiveness of RCA so that I can use the most efficient method of amplification for the cell-based experiments. This builds on the T4 experiments and the circular product production. I will be using that circular product to analyze the effectiveness of RCA.
Linear Product Production (Continued from yesterday)
- TBE gel
- Mix 64 uL TBE and 16 uL 6X dye
- Aliquot 10 uL on to parafilm per sample/ladder
- Add 2 uL reaction, 1 uL (10 uM) controls, and 1.5 uL ladder to appropriate aliquot File:GelLanes-20170325-LinearProduct.png
- Add 10 uL mix to gel lanes
- Run gel for 24 minutes at 230V
- Stain with 2 uL SYBR gold for 3 minutes
- Rinse and image in gel doc