Daniel:Notebook/ComboLock/2017-4-12: Difference between revisions

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{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
|- style="font-size:12pt;font-weight:bold" align="center"
|style="background-color:#8DB4E2" width="191" height="30" | Reagent
|style="background-color:#8DB4E2" width="250" height="30" | Reagent
|style="background-color:#8DB4E2" width="65" | Single Rxn Vol
|style="background-color:#8DB4E2" width="85" | Single Rxn Vol
|style="background-color:#95B3D7" width="65" | Master Mix (13.2X)
|style="background-color:#95B3D7" width="85" | Master Mix (13.2X)


|- style="font-size:12pt"
|- style="font-size:12pt"

Revision as of 15:11, 12 April 2017

RCA Double Deuce

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Protocol

  1. Bglii digestion
    1. Make the following reactions; Do not add Bglii yet
    2. Reagent Single Rxn Vol Master Mix (13.2X)
      RCA Rxn 5 0
      Bglii Cutter Primer + (100 uM) 2 26.4
      10X Buffer 3.1 2 26.4
      Bglii 1 0
      nfH2O 10 132
      Total 20 184.8
    3. Aliquot 14 uL master mix into new tube
    4. Add 5 uL appropriate sample
    5. Use the following thermocycler program
      1. 2 hr 37C; When this cycle starts add 1 uL Bglii
      2. Heat kill with 65C for 20 min