AlanFung:Protocol/Cell Counting: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 (New page: '''Cell Counting''' *Material Cell counter Small cup Balance solution 5mL pipette pipette aid P200 pipette P200 pipette tips *Overview Prime Amperture Suspend Cells Perform Cell Count) |
>Alan6017518 No edit summary |
||
(3 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
'''Cell Counting''' | '''Cell Counting''' | ||
*Material | *Material | ||
Cell counter | |||
Small cup | Cell counter | ||
Balance solution | Small cup | ||
5mL pipette | Balance solution | ||
5mL pipette | |||
P200 pipette | Pipette aid | ||
P200 pipette tips | P200 pipette | ||
P200 pipette tips | |||
*Overview | *Overview | ||
Prime Amperture | |||
Suspend Cells | Prime Amperture | ||
Perform Cell Count | Suspend Cells | ||
Perform Cell Count | |||
*Protocol | |||
Prime Amperature | |||
Turn on cell counter | |||
Fill small cup with 10mL balance solution | |||
Replace cleaning solution cup with | |||
*Prepare cups with 10ml balance solution(1cup/sample) | |||
*Prepare single cell suspension by repeat pipetting | |||
*Aspirate 100uL of single cell suspension into cup | |||
*Swirl cup | |||
*Place cup into holder | |||
*Hit <Setup> then <Enter> |
Latest revision as of 01:03, 17 June 2009
Cell Counting
- Material
Cell counter Small cup Balance solution 5mL pipette Pipette aid P200 pipette P200 pipette tips
- Overview
Prime Amperture Suspend Cells Perform Cell Count
- Protocol
Prime Amperature
Turn on cell counter Fill small cup with 10mL balance solution Replace cleaning solution cup with
- Prepare cups with 10ml balance solution(1cup/sample)
- Prepare single cell suspension by repeat pipetting
- Aspirate 100uL of single cell suspension into cup
- Swirl cup
- Place cup into holder
- Hit <Setup> then <Enter>