AlanFung:Protocol/Cell Counting: Difference between revisions

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(New page: '''Cell Counting''' *Material Cell counter Small cup Balance solution 5mL pipette pipette aid P200 pipette P200 pipette tips *Overview Prime Amperture Suspend Cells Perform Cell Count)
 
>Alan6017518
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'''Cell Counting'''
'''Cell Counting'''
*Material
*Material
Cell counter
 
Small cup
Cell counter
Balance solution
Small cup
5mL pipette
Balance solution
pipette aid
5mL pipette
P200 pipette
Pipette aid
P200 pipette tips
P200 pipette
P200 pipette tips


*Overview
*Overview
Prime Amperture
 
Suspend Cells
Prime Amperture
Perform Cell Count
Suspend Cells
Perform Cell Count
 
*Protocol
Prime Amperature
Turn on cell counter
Fill small cup with 10mL balance solution
Replace cleaning solution cup with
 
*Prepare cups with 10ml balance solution(1cup/sample)
*Prepare single cell suspension by repeat pipetting
*Aspirate 100uL of single cell suspension into cup
*Swirl cup
*Place cup into holder
*Hit <Setup> then <Enter>

Latest revision as of 01:03, 17 June 2009

Cell Counting

  • Material
Cell counter
Small cup
Balance solution
5mL pipette
Pipette aid
P200 pipette
P200 pipette tips
  • Overview
Prime Amperture
Suspend Cells
Perform Cell Count
  • Protocol

Prime Amperature

Turn on cell counter
Fill small cup with 10mL balance solution
Replace cleaning solution cup with
  • Prepare cups with 10ml balance solution(1cup/sample)
  • Prepare single cell suspension by repeat pipetting
  • Aspirate 100uL of single cell suspension into cup
  • Swirl cup
  • Place cup into holder
  • Hit <Setup> then <Enter>