AlanFung:Protocol/Cell Counting: Difference between revisions

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Line 25: Line 25:
*Prepare single cell suspension by repeat pipetting
*Prepare single cell suspension by repeat pipetting
*Aspirate 100uL of single cell suspension into cup
*Aspirate 100uL of single cell suspension into cup
*
*Swirl cup
*Place cup into holder
*Hit <Setup> then <Enter>

Latest revision as of 01:03, 17 June 2009

Cell Counting

  • Material
Cell counter
Small cup
Balance solution
5mL pipette
Pipette aid
P200 pipette
P200 pipette tips
  • Overview
Prime Amperture
Suspend Cells
Perform Cell Count
  • Protocol

Prime Amperature

Turn on cell counter
Fill small cup with 10mL balance solution
Replace cleaning solution cup with
  • Prepare cups with 10ml balance solution(1cup/sample)
  • Prepare single cell suspension by repeat pipetting
  • Aspirate 100uL of single cell suspension into cup
  • Swirl cup
  • Place cup into holder
  • Hit <Setup> then <Enter>