AlanFung:Protocol/Cell Counting: Difference between revisions
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*Prepare single cell suspension by repeat pipetting | *Prepare single cell suspension by repeat pipetting | ||
*Aspirate 100uL of single cell suspension into cup | *Aspirate 100uL of single cell suspension into cup | ||
* | *Swirl cup | ||
*Place cup into holder | |||
*Hit <Setup> then <Enter> |
Latest revision as of 01:03, 17 June 2009
Cell Counting
- Material
Cell counter Small cup Balance solution 5mL pipette Pipette aid P200 pipette P200 pipette tips
- Overview
Prime Amperture Suspend Cells Perform Cell Count
- Protocol
Prime Amperature
Turn on cell counter Fill small cup with 10mL balance solution Replace cleaning solution cup with
- Prepare cups with 10ml balance solution(1cup/sample)
- Prepare single cell suspension by repeat pipetting
- Aspirate 100uL of single cell suspension into cup
- Swirl cup
- Place cup into holder
- Hit <Setup> then <Enter>