Daniel:Notebook/PosSequencing/2017-4-28: Difference between revisions

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==Images and Analysis==
==Images and Analysis==
===Field 4===


<gallery perrow=3 heights=300px widths=300px caption="Field 4 Images">
<gallery perrow=3 heights=300px widths=300px caption="Field 4 Images">
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</gallery>  
</gallery>  


Field 4 had the best results and is worth a summary. There are 5 fluorescent spots. Not counting Cy3, which shouldn't be there and has extra signal that is not present in the other channels. Two of the molecules are too large to be a single bead, which should be ~1 um. However, 3 of the signals are that size. Two have A488 signal and one has Cy5 signal.
===Other Fields===
<gallery perrow=3 heights=300px widths=300px caption="Assorted Field Images">
File:2017-04-28-emPCR-Field1Overlay.jpg|Field 1 overlay; Note that this one is brightfield/A488 only. The Cy5 channel was weak on this one, and Cy3 wasn't measured
File:2017-04-28-emPCR-Field2-Overlay-Cy3-GFP-Cy5.jpg|Cy3 image (Note: No Cy3 was used, therefore there shouldn't be a Cy3 signal)
File:20170428-emPCR2_w3Cy5-4.jpg|Cy5 image; barcode oligo v3
File:2017-04-28-emPCR-Field4-Overlay-Cy3-GFP-Cy5.jpg|Dye overlays; Cy3-white; A488-green; Cy5-Red
File:20170428-Field4Overlay-Measured.png|Dye overlays, individual bubbles measured as 2.5 um each (2 A488, 1 Cy5)
</gallery>




[[Category:PosSeq]] [[Category:20170426]]
[[Category:PosSeq]] [[Category:20170426]]

Revision as of 19:25, 28 April 2017

Emulsion PCR (Started Yesterday)

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Images and Analysis

Field 4

Field 4 had the best results and is worth a summary. There are 5 fluorescent spots. Not counting Cy3, which shouldn't be there and has extra signal that is not present in the other channels. Two of the molecules are too large to be a single bead, which should be ~1 um. However, 3 of the signals are that size. Two have A488 signal and one has Cy5 signal.

Other Fields