Daniel:Notebook/ComboLock/2017-5-2: Difference between revisions
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<li>Size Select TBE Gel</li> | <li>Size Select TBE Gel</li> | ||
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<gallery perrow=2 heights=300px widths=300px> | |||
File:2017-05-02-ProductionRun-SizeSelection.png|Pre Selection | |||
File:2017-05-02-ProductionRun-SizeSelection-After.png|Post Selection | |||
</gallery> | |||
</ol></ol> | </ol></ol> |
Revision as of 17:00, 2 May 2017
Production Run (Started Friday 4/28)
Library Prep
- Qiaquick Purification
- Combine 20 uL each sample into a 1.5 mL tube following matrix
- Add 200 uL (5X) PB (binding buffer) to sample
- Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
- Add 700 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
- Repeat above step with 400 uL PE buffer
- Dry spin column for 2 minutes at 14000 rpm; discard flow through
- Let stand with cover open in fume hood for ~5 minutes
- Transfer column to a new 1.5 mL eppendorf tube
- Add 30 uL nfH2O to column
- Spin for 1 minute at 14000 rpm
- Size Select TBE Gel
- 2017-05-02-ProductionRun-SizeSelection.png
Pre Selection
- 2017-05-02-ProductionRun-SizeSelection-After.png
Post Selection
Tube | Sample 1 | Sample 2 | Indexes |
1X | 1AX | 1BX | 20 |
5AX | 5AX | nfH2O | 24 |
1Y | 1AY | 1BY | 25 |
5AY,4AY | 5AY | 4AY | 26,27 |