AlanFung:Lab Notebook/DNA Methylation: Difference between revisions
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>Alan6017518 (New page: '''DNA Methylation''' *Where 5 position of the pyrimidine ring of cytosines in the context of the dinucleotide sequence CpG *How *Response to stimuli diet toxins *Aberrant DNA methyla...) |
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'''DNA Methylation''' | '''DNA Methylation''' | ||
*Where | |||
5 position of the pyrimidine ring of cytosines in the context of the dinucleotide sequence CpG | *What is DNA Methylation | ||
It is the only genetically programmed DNA modification in mammals | |||
''Where'' | |||
*Almost exclusively found on the 5 position of the pyrimidine ring of cytosines in the context of the dinucleotide sequence CpG | |||
*CpG Island (G+C content of more than 50%; observed versus expected ratio for the occurrence of CpGs of more than 0.6; minimum size 200bp) | |||
*Frequency | |||
The majority (75%) of CpG dinucleotides throughout mammalian genomes are methylated | |||
*How | *How | ||
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Used to detect cancer at very early stages | Used to detect cancer at very early stages | ||
*Classify tumors | *Classify tumors | ||
*predict and monitor the response to antineoplastic treatment | |||
* | |||
''DNA Methylation Changes in Cancer'' | |||
*Tumor formation leads to a global loss of DNA methylation(hypomethylation) | |||
initiating and propagate |
Latest revision as of 21:52, 2 April 2009
DNA Methylation
- What is DNA Methylation
It is the only genetically programmed DNA modification in mammals
Where
- Almost exclusively found on the 5 position of the pyrimidine ring of cytosines in the context of the dinucleotide sequence CpG
- CpG Island (G+C content of more than 50%; observed versus expected ratio for the occurrence of CpGs of more than 0.6; minimum size 200bp)
- Frequency
The majority (75%) of CpG dinucleotides throughout mammalian genomes are methylated
- How
- Response to stimuli
diet toxins
- Aberrant DNA methylation
Leads to cancer - where genome wide hypomethylation coincides with gene-specific hypermethylation
- Usage
The epigenetic mechanisms controlling and modulating gene expression through chromatin structure Closely interacts with histone modifications and chromatin-remodeling complexes to form the genomic chromatin landscape Essential for proper mammalian development Crucial for imprinting Plays a role in maintaining genomic stability and dosage compensation Used to detect cancer at very early stages
*Classify tumors *predict and monitor the response to antineoplastic treatment *
DNA Methylation Changes in Cancer
- Tumor formation leads to a global loss of DNA methylation(hypomethylation)
initiating and propagate