Daniel:Notebook/ComboLock/2017-5-16: Difference between revisions

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| width="120" | Missing UMIs (%)
| width="120" | Missing UMIs (%)
| width="120" | Poisson Lambda
| width="120" | Poisson Lambda
| width="155" | Poissonian E[Missing UMIs] (%)
| width="155" | Poissonian E[0] (%)
| width="125" | UMI Missing Enrichment
| width="125" | UMI Missing Enrichment



Revision as of 22:27, 16 May 2017

Production Run (Started Friday 4/28; Libary Prep)

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Sequencing Results

These results are from the sequencing run performed 5/8/2017.

Read Statistics

Sample Condition Reads Aligned Reads Unaligned Pct Aligned Missing UMIs (%) Poisson Lambda Poissonian E[0] (%) UMI Missing Enrichment
1X NEB Sample 254728 21861 92.1% 34.17 3.89 2.0 16.7
5AX NEB Positive Control 119220 10206 92.1% 31.2 1.82 16.2 1.9
1Y TF Sample 197959 23195 89.5% 42.53 3.02 4.9 8.7
5AY TF Positive Control 63074 6955 90.1% 51.37 0.96 38.3 1.3
4AY TF Dephosphorylated 133660 15412 89.7% 72.47 2.03 13.1 5.5
NA Randomly Generated 250,000 UMIs     2.26 3.81 2.21 1.0

UMI Counting