Daniel:Notebook/PosSequencing/2017-5-17: Difference between revisions
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'''Alkaline Lysis Solution''' | '''Alkaline Lysis Solution''' | ||
*80 ul Nuclease free | *80 ul Nuclease free H<sub>2</sub>O. | ||
*8 ul 5 M KOH. | *8 ul 5 M KOH. | ||
*10 ul 1 M DTT. | *10 ul 1 M DTT. |
Revision as of 18:23, 17 May 2017
Bead Binding Test 3
Trying to test the bead binding and denaturation on the beads again. This time we'll use high pH as the denaturant. From the paper above it says the streptavidin bond is stable to about pH 11, and according to this website DNA denaturation occurs at pH>10. I will use the alkaline lysis solution from Andrew's notes
Alkaline Lysis Solution
- 80 ul Nuclease free H2O.
- 8 ul 5 M KOH.
- 10 ul 1 M DTT.
- 2 ul 0.5 M EDTA
Neutralization Solution
- 3 mL H20
- 3 mL 2M Tris-HCl
- 4 mL 1N HCl