Daniel:Notebook/PosSequencing/2017-5-22: Difference between revisions
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>Djacobse (Created page with "=Single Stranded Probe Binding Test (Started Friday May 19)= Back to Calendar ==Protocol (Conti...") |
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[[Daniel:Notebook/PosSequencing|Back to Calendar]] | [[Daniel:Notebook/PosSequencing|Back to Calendar]] | ||
==Protocol (Continued from [[Daniel:Notebook/PosSeq/2017-5- | ==Protocol (Continued from [[Daniel:Notebook/PosSeq/2017-5-20|Saturday May 20]])== | ||
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Revision as of 16:07, 22 May 2017
Single Stranded Probe Binding Test (Started Friday May 19)
Protocol (Continued from Saturday May 20)
- Dye hybridization
- Mix 20 uL ssDNA (lambda digested) and 20 uL dsDNA (original, non-digested template)
- Add 1 uL appropriate (10 uM) dye, A488-dc6 for sample 1 and Cy5-dc6 for sample 3
- Incubate in the dark (RT) for 15 minutes
- Magnet pulldown, remove supernatant and add 40 uL 2X SSC buffer
- Add 15 uL to a slide for analysis
- Seal coverslip with nail polish and wait approximately 30 minutes to dry
- Image on microscope