Daniel:Notebook/ComboLock/2017-6-20: Difference between revisions
Jump to navigation
Jump to search
>Djacobse |
>Djacobse |
||
Line 28: | Line 28: | ||
1.22 ug/mL | 1.22 ug/mL | ||
[[Category:ComboLock]] [[category: | [[Category:ComboLock]] [[category:20170614]] |
Revision as of 23:05, 22 June 2017
Production Run (Started Wednesday 6/14)
Library Prep (Started Yesterday)
- Ethanol Precipitation
- Remove from -80C and centrifuge for 30 minutes at 12000rpm at 4C
- Remove supernatant and add 700 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and dry in vacuum centrifuge for 10 minutes
- Resuspend and pool samples in 30 uL nfH2O
- Qubit Measurement
- Mix 597 uL Qubit buffer with 3 uL dye
- For standards, add 190 uL buffer mix and 10 uL standard to a Qubit tube
- For sample, add 199 uL buffer mix and 1 uL sample to a Qubit tube
- Let sit in the dark 5 minutes
- Measure in Qubit using new standards
Library DEJ20170610-CLAbPC-01
1.22 ug/mL