Daniel:Notebook/ComboLock/2017-7-11: Difference between revisions

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>Djacobse
(Created page with "=Oligo+Protein Test (Started Thursday July 6)= Back to Calendar ==Library Prep (Started Daniel:Notebo...")
 
>Djacobse
Line 9: Line 9:
<li>Ethanol Precipitation</li>
<li>Ethanol Precipitation</li>
<ol type="A">
<ol type="A">
<li>Remove from -80C and centrifuge for 30 minutes at 12000rpm at 4C</li>
<li>Remove supernatant and add 700 uL chilled 70% EtOH</li>
<li>Centrifuge for 12 minutes at 12000rpm at 4C</li>
<li>Remove supernatant and dry in vacuum centrifuge for 10 minutes</li>
<li>Resuspend and pool samples in 35 uL nfH<sub>2</sub>O</li>
</ol>
<li>Qubit Measurement</li>
<ol type="A">
<li>Mix 597 uL Qubit buffer with 3 uL dye</li>
<li>For standards, add 190 uL buffer mix and 10 uL standard to a Qubit tube</li>
<li>For sample, add 199 uL buffer mix and 1 uL sample to a Qubit tube</li>
<li>Let sit in the dark 5 minutes</li>
<li>Measure in Qubit using new standards</li>
</ol>
</ol>
</ol>
</ol>


'''DEJ20170711-MixPC-01'''5.13 ug/mL (ng/uL)
'''DEJ20170711-MixPC-01''': 5.13 ug/mL (ng/uL)

Revision as of 18:21, 11 July 2017

Oligo+Protein Test (Started Thursday July 6)

Back to Calendar


Library Prep (Started Yesterday)

  1. Ethanol Precipitation
    1. Remove from -80C and centrifuge for 30 minutes at 12000rpm at 4C
    2. Remove supernatant and add 700 uL chilled 70% EtOH
    3. Centrifuge for 12 minutes at 12000rpm at 4C
    4. Remove supernatant and dry in vacuum centrifuge for 10 minutes
    5. Resuspend and pool samples in 35 uL nfH2O
  2. Qubit Measurement
    1. Mix 597 uL Qubit buffer with 3 uL dye
    2. For standards, add 190 uL buffer mix and 10 uL standard to a Qubit tube
    3. For sample, add 199 uL buffer mix and 1 uL sample to a Qubit tube
    4. Let sit in the dark 5 minutes
    5. Measure in Qubit using new standards

DEJ20170711-MixPC-01: 5.13 ug/mL (ng/uL)