Daniel:Notebook/PosSequencing/2017-7-12: Difference between revisions

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=emPCR 9-Concentration Comparison=


[[Category:PosSeq]] [[Category:20170706]]
[[Category:PosSeq]] [[Category:20170706]]

Revision as of 21:42, 12 July 2017

emPCR8 (Started Thursday July 6)

Back to Calendar

Protocol (From Yesterday)

  1. Dye hybridization
    1. Mix 20 uL ssDNA (lambda digested) and 20 uL 4X SSC buffer
    2. Incubate in the dark at 70C for 5 minutes
    3. Incubate at 55C for 20 minutes
    4. Add 1 uL appropriate (10 uM) dye, A488-dc6 for sample 1 and Cy5-dc6 for sample 3
    5. Incubate in the dark (RT) for 15 minutes
    6. Magnet pulldown, remove supernatant and add 40 uL 2X SSC buffer
    7. Add 15 uL to a slide for analysis
    8. Seal coverslip with nail polish and wait approximately 30 minutes to dry

Results

emPCR 9-Concentration Comparison