Sam:LabNotes/Human/2009-5-13: Difference between revisions

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>Sam Chiang
(New page: ='''Library transfering and shipping for shotgun sequencing'''= *Results of glycerol stock plate from 5-12-09 **Check the plate growing status on black backgroud. Almost all wells have co...)
 
>Sam Chiang
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='''Library transfering and shipping for shotgun sequencing'''=
='''Library transfering and shipping for shotgun sequencing'''=


*Results of glycerol stock plate from 5-12-09
*Results of glycerol stock plate from 5-12-09:
**Check the plate growing status on black backgroud. Almost all wells have colonies growing and generate smear LB media.
**After 14hr incubation, check the plate growing status on black backgroud (AM 9:00). Almost all wells have colonies growing and generate smear LB media.


*Two glycerol stock plates (5/7/09, 5/12/09) were combined together and sealed with alumina film.
*Two glycerol stock plates (5/7/09, 5/12/09) were combined together and sealed with alumina film.

Revision as of 23:33, 13 May 2009

Library transfering and shipping for shotgun sequencing

  • Results of glycerol stock plate from 5-12-09:
    • After 14hr incubation, check the plate growing status on black backgroud (AM 9:00). Almost all wells have colonies growing and generate smear LB media.
  • Two glycerol stock plates (5/7/09, 5/12/09) were combined together and sealed with alumina film.
Colonies contaminated with B3 samples  Column 1-12 Row A-D, 48 wells in total
Colonies contaminated wiht D5 samples  Column 1-12 Row E-H, 48 wells in total
  • The glycerol stock plate was stored in -80 to freeze for 4hr
  • The frozen plate was shipped with dry ice via FedEx to Agencourt at 4:00 PM (FedEx track #865950992390)