Alice:LabNotes/2007-12-12: Difference between revisions
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Note: 20uL SYBEsafe was added to the agrose gel, so no further staining will be needed.<br> | |||
[[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] | [[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] | ||
<br><b> | <br><b> | ||
There were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice. <br> | There were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice. <br> | ||
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected. </b> | Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected. </b> |
Revision as of 19:29, 8 January 2008
Agarose gel on post-PCR product of PGP1Ver3 Primers
' | well 1 | well 2-13 | |||||||
Low Mass bp Ladder | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
Novex 5x TBE loading dye | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | |
post-PCR sample | 0 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | |
Note: 20uL SYBEsafe was added to the agrose gel, so no further staining will be needed.
File:ZhangLab 2 2007-12-12 13hr 32min.jpg
There were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice.
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected.