Sam:LabNotes/Human/2009-5-13: Difference between revisions

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*The frozen plate was shipped with dry ice via FedEx to Agencourt at 4:00 PM (FedEx track #865950992390)
*The frozen plate was shipped with dry ice via FedEx to Agencourt at 4:00 PM (FedEx track #865950992390)
==Results==
*[[Media:051309-MDA_shotgun_06012009-all.txt|Sanger sequence results (vector sequence masked)]]
*[[Media:Blat summary -result1.xls|Blat results (against to human genome)]]
*[[Media:BLAST reuslt for low-quality sequence - result 2.txt|Additional blast results for low-quality sequences]]

Latest revision as of 08:13, 9 June 2009

Library transfering and shipping for shotgun sequencing[edit]

  • Results of glycerol stock plate from 5-12-09:
    • After 14hr incubation, check the plate growing status on black backgroud (AM 9:00). Almost all wells have colonies growing and generate smear LB media.
  • Two glycerol stock plates (5/7/09, 5/12/09) were combined together and sealed with alumina film.
Colonies contaminated with B3 samples  Column 1-12 Row A-D, 48 wells in total
Colonies contaminated with D5 samples  Column 1-12 Row E-H, 48 wells in total
  • The glycerol stock plate was stored in -80 to freeze for 4hr
  • The frozen plate was shipped with dry ice via FedEx to Agencourt at 4:00 PM (FedEx track #865950992390)

Results[edit]