Alice:LabNotes/2007-12-12: Difference between revisions
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<b>Agarose gel on post-PCR product of PGP1Ver3 Primers</b><br> | <b>Agarose gel on post-PCR product of PGP1Ver3 Primers</b><br> | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''well 1''' | |||
| align="center" style="background:#f0f0f0;"|'''well 2-13''' | |||
|- | |||
| Low Mass bp Ladder ||3 ul ||0 ul ||0 ul||0 ul||0 ul||0 ul||0 ul||0 ul|| | |||
|- | |||
| Novex 5x TBE loading dye || 2 ul|| 2 ul|| 2 ul|| 2 ul|| 2 ul|| 2 ul||2 ul||2 ul|| | |||
|- | |||
| post-PCR sample ||0 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul || | |||
|- | |||
| | |||
|} | |||
<br> | |||
Note: 20uL SYBEsafe was added to the agrose gel, so no further staining will be needed.<br> | |||
[[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] | [[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] | ||
<br><b> | |||
There was a mistake on this experiment: the reaction was not setup on ice, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. <br> | |||
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected. </b> |
Latest revision as of 21:40, 8 January 2008
Agarose gel on post-PCR product of PGP1Ver3 Primers
' | well 1 | well 2-13 | |||||||
Low Mass bp Ladder | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
Novex 5x TBE loading dye | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | |
post-PCR sample | 0 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | |
Note: 20uL SYBEsafe was added to the agrose gel, so no further staining will be needed.
File:ZhangLab 2 2007-12-12 13hr 32min.jpg
There was a mistake on this experiment: the reaction was not setup on ice, therefore the reaction would not proceed as expected, whereas the reactions will start at different times.
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected.