Alice:LabNotes/2007-12-12: Difference between revisions

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Note: 20uL SYBEsafe was added to the agrose gel, so no further staining will be needed.<br>
[[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]]
[[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]]
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Note: there were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice. <br>
There was a mistake on this experiment: the reaction was not setup on ice, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. <br>
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected. </b>
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected. </b>

Latest revision as of 21:40, 8 January 2008

Agarose gel on post-PCR product of PGP1Ver3 Primers

' well 1 well 2-13
Low Mass bp Ladder 3 ul 0 ul 0 ul 0 ul 0 ul 0 ul 0 ul 0 ul
Novex 5x TBE loading dye 2 ul 2 ul 2 ul 2 ul 2 ul 2 ul 2 ul 2 ul
post-PCR sample 0 ul 3 ul 3 ul 3 ul 3 ul 3 ul 3 ul 3 ul


Note: 20uL SYBEsafe was added to the agrose gel, so no further staining will be needed.
File:ZhangLab 2 2007-12-12 13hr 32min.jpg
There was a mistake on this experiment: the reaction was not setup on ice, therefore the reaction would not proceed as expected, whereas the reactions will start at different times.
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected.