Kun:LabNotes/ASE/2007-12-20: Difference between revisions

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Line 11: Line 11:
     Template:          1ul
     Template:          1ul
     2X Taq Mastermix: 15ul
     2X Taq Mastermix: 15ul
     2uM dNTP:          3ul
     2uM primers        3ul
     H2O:              11ul
     H2O:              11ul


94C 3min -> (94C 30sec -> 55C 30sec -> 72C 30sec) x 40 -> 72C 3min -> 4C hold.
94C 3min -> (94C 30sec -> 55C 30sec -> 72C 30sec) x 40 -> 72C 3min -> 4C hold.
   [[Image:2007-12-20-Hues6ver2-PCR.png|400px]]
   [[Image:2007-12-20-Hues6ver2-PCR.png|400px]]

Latest revision as of 00:53, 4 January 2008

Exp.1 PCR on Hues6, GM20431, GC1F, GC1EP cDNA using the Hues6Ver2 primer set[edit]

The primer plate I ordered came in today. I made 10uM working primer mix by adding 5ul of forward and reverse primers into 90ul of ddH2O.

Template:

  • Hues6 cDNA (04/18/07) 107ng/ul
  • GM20431 dsCDNA (05/22/07) 62ng/ul
  • GC1F sscDNA (06/05/07) 54ng/ul
  • GC1EP sscDNA(06/15/07) 213ng/ul

Make dilution to 5ng/ul

    Template:          1ul
    2X Taq Mastermix: 15ul
    2uM primers        3ul
    H2O:              11ul

94C 3min -> (94C 30sec -> 55C 30sec -> 72C 30sec) x 40 -> 72C 3min -> 4C hold.

  File:2007-12-20-Hues6ver2-PCR.png