AlanFung:Protocol/FACScan: Difference between revisions
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==Cytometer Appearance (Bottom Panel)== | ==Cytometer Appearance (Bottom Panel)== | ||
------------- --------- -------------- | ------------- --------- -------------- | ||
| Sheath | |Sheath | | Waste | | | Sheath | |Sheath | | Waste | | ||
| Tank | |Filter | | Tank | | | Tank | |Filter | | Tank | | ||
------------- --------- -------------- | ------------- --------- -------------- | ||
==Cytometer Setup== | ==Cytometer Setup== |
Revision as of 01:47, 18 June 2009
FACScan (Becton Dickinson)
Cytometer Appearance (Bottom Panel)
------------- --------- -------------- | Sheath | |Sheath | | Waste | | Tank | |Filter | | Tank | ------------- --------- --------------
Cytometer Setup
- Turn on both the cytometer and the computer
- Open up the bottom panel on the cytometer
- Make sure the system is pressurized
(Vent valve should be in the upwards position) (Sheath tank should be tight and non-movable) (No gap between the sheath tank and the top of the rack)