AlanFung:Protocol/FACScan: Difference between revisions

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==Cytometer Appearance (Bottom Panel)==
==Cytometer Appearance (Bottom Panel)==


  -------------  ---------  --------------
  -------------  --------------  --------------
  | Sheath    |  |Sheath |  |  Waste    |
  | Sheath    |  |.2um Sheath |  |  Waste    |
  | Tank      |  |Filter |  |  Tank    |
  | Tank      |  |Filter     |  |  Tank    |
  -------------  ---------  --------------
  -------------  --------------  --------------


==Cytometer Setup==
==Cytometer Setup==

Revision as of 01:48, 18 June 2009

FACScan (Becton Dickinson)

Cytometer Appearance (Bottom Panel)

-------------   --------------  --------------
| Sheath    |   |.2um Sheath |  |   Waste    |
| Tank      |   |Filter      |  |   Tank     |
-------------   --------------  --------------

Cytometer Setup

  • Turn on both the cytometer and the computer
  • Open up the bottom panel on the cytometer
  • Make sure the system is pressurized
(Vent valve should be in the upwards position)
(Sheath tank should be tight and non-movable)
(No gap between the sheath tank and the top of the rack)