AlanFung:Protocol/FACScan: Difference between revisions
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(Sheath tank should be tight and non-movable) | (Sheath tank should be tight and non-movable) | ||
(No gap between the sheath tank and the top of the rack) | (No gap between the sheath tank and the top of the rack) | ||
===Purge bubbles trapped in the filter=== | |||
*Pull white cap off from the tube coming out from the sheath filter | |||
* |
Revision as of 01:50, 18 June 2009
FACScan (Becton Dickinson)
Cytometer Appearance (Bottom Panel)
------------- -------------- -------------- | Sheath | |.2um Sheath | | Waste | | Tank | |Filter | | Tank | ------------- -------------- --------------
Cytometer Setup
- Turn on both the cytometer and the computer
- Open up the bottom panel on the cytometer
- Make sure the system is pressurized
(Vent valve should be in the upwards position) (Sheath tank should be tight and non-movable) (No gap between the sheath tank and the top of the rack)
Purge bubbles trapped in the filter
- Pull white cap off from the tube coming out from the sheath filter