AlanFung:Protocol/FACScan: Difference between revisions
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*Release the water with bubbles into the small waste beaker until no bubbles are seen | *Release the water with bubbles into the small waste beaker until no bubbles are seen | ||
===Removal of bubbles from flow cell== | ===Removal of bubbles from flow cell=== | ||
*Open up the middle panel on the cytometer, locate the flow cell (a small window with fluid in it) | *Open up the middle panel on the cytometer, locate the flow cell (a small window with fluid in it) | ||
*Swing the supporting arm to the side | *Swing the supporting arm to the side | ||
Line 30: | Line 30: | ||
*Leave the control knob on <Fill> | *Leave the control knob on <Fill> | ||
*Close the middle panel | *Close the middle panel | ||
Revision as of 02:01, 18 June 2009
FACScan (Becton Dickinson)
Cytometer Appearance (Bottom Panel)
------------- -------------- -------------- | Sheath | |.2um Sheath | | Waste | | Tank | |Filter | | Tank | ------------- -------------- --------------
Cytometer Setup
- Turn on both the cytometer and the computer
- Open up the bottom panel on the cytometer
- Make sure the system is pressurized
(Vent valve should be in the upwards position) (Sheath tank should be tight and non-movable) (No gap between the sheath tank and the top of the rack)
Purge bubbles trapped in the filter
- Prepare a small waster beaker located near the sink
- Squeeze the tube and pull white cap off from the tube coming out from the sheath filter
- Release the water with bubbles into the small waste beaker until no bubbles are seen
Removal of bubbles from flow cell
- Open up the middle panel on the cytometer, locate the flow cell (a small window with fluid in it)
- Swing the supporting arm to the side
- Take out tube containing MilliQ H2O
- Turn knob on the control panel to <Drain> in order to drain the flow cell (Notice the fluid level drops)
- Once the flow cell is drained, turn the knob to <Fill> (Notice the fluid level rises)
- Repeat the drain and fill procedure 3 times or until no bubbles are seen on the wall of the flow cell
- Leave the control knob on <Fill>
- Close the middle panel