Kun:LabNotes/ExonomeSeq/2007-12-23: Difference between revisions

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Line 5: Line 5:
     100uM AmpFV6.2Sol    0.4ul
     100uM AmpFV6.2Sol    0.4ul
     100uM AmpRV6.2Sol    0.4ul
     100uM AmpRV6.2Sol    0.4ul
     50X SYBG I            0.2ul
     50X SYBG I            0.4ul
     JumpStart Taq          1ul
     JumpStart Taq          1ul
     H2O                    79ul
     H2O                    79ul


94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.

Revision as of 01:21, 24 December 2007

Exp.1 PCR on the capturing reaction started on 12/21

    Template:              10ul
    10X JumpStart buffer:   9ul
    25mM dNTP               1ul
    100uM AmpFV6.2Sol     0.4ul
    100uM AmpRV6.2Sol     0.4ul
    50X SYBG I            0.4ul
    JumpStart Taq           1ul
    H2O                    79ul

94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.