AlanFung:Protocol/Qubit: Difference between revisions
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>Alan6017518 (New page: ==Qubit Fluorometer Protocol== ==Samples & Materials== *Qubit Assay Tubes (500, Invitrogen Cat. no.Q32856) or Axygen PCR-05-C tubes (VWR, part number 10011-830) ==Calibrating the Qubit Flu...) |
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==Qubit Fluorometer Protocol== | ==Qubit Fluorometer Protocol== | ||
==Samples & Materials== | ==Samples & Materials== | ||
*Qubit fluorometer Invitrogen Cat. Q32857 | |||
*Qubit Assay Tubes (500, Invitrogen Cat. no.Q32856) or Axygen PCR-05-C tubes (VWR, part number 10011-830) | *Qubit Assay Tubes (500, Invitrogen Cat. no.Q32856) or Axygen PCR-05-C tubes (VWR, part number 10011-830) | ||
*Quant-IT DSDNA BR Assay Kit Invitrogen Cat. Q32850 | |||
*Quant-IT DSDNA HS Assay Kit Invitrogen Cat. Q32851 | |||
*Quant-IT RNA Assay Kit Invitrogen Cat. Q32852 | |||
*Quant-IT Protein Assay Kit Invitrogen Cat. Q33211 | |||
[[Image:Qubit1.jpg]] | |||
[[Image:Qubit2.jpg]] | |||
==Calibrating the Qubit Fluorometer== | ==Calibrating the Qubit Fluorometer== | ||
===Running a New Calibration=== | ===Running a New Calibration=== | ||
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*Insert standard #2 | *Insert standard #2 | ||
*Press <Go> | *Press <Go> | ||
* | |||
===Using the Last Calibration=== | |||
*Highlight <Use Last Calibration> | |||
*Press <Go> | *Press <Go> | ||
==Reading Samples== | |||
*Insert sample | |||
*Press <Go> | |||
*Read the result on the display | |||
*Record the reading | |||
*Remove the sample form the instrument | |||
*Insert the next sample | |||
*Press <Go> | |||
*Repeat until all samples have been read | |||
==Calculation the Concentration of your Sample== | |||
*Qubuit fluorometer gives values in either ug/ml or ng/ml | |||
*Concentration of your sample = QF value x (200/x) | |||
*where QF = the value given by the Qubit fluorometer | |||
x = the number of microliters of sample you added to the assay tube | |||
*This equation generates a result with the same units as the value given by the Qubit fluorometer |
Latest revision as of 22:04, 30 June 2009
Qubit Fluorometer Protocol[edit]
Samples & Materials[edit]
- Qubit fluorometer Invitrogen Cat. Q32857
- Qubit Assay Tubes (500, Invitrogen Cat. no.Q32856) or Axygen PCR-05-C tubes (VWR, part number 10011-830)
- Quant-IT DSDNA BR Assay Kit Invitrogen Cat. Q32850
- Quant-IT DSDNA HS Assay Kit Invitrogen Cat. Q32851
- Quant-IT RNA Assay Kit Invitrogen Cat. Q32852
- Quant-IT Protein Assay Kit Invitrogen Cat. Q33211
File:Qubit1.jpg File:Qubit2.jpg
Calibrating the Qubit Fluorometer[edit]
Running a New Calibration[edit]
- Insert standard #1
- Press <Go>
- Wait for ~5s
- Insert standard #2
- Press <Go>
Using the Last Calibration[edit]
- Highlight <Use Last Calibration>
- Press <Go>
Reading Samples[edit]
- Insert sample
- Press <Go>
- Read the result on the display
- Record the reading
- Remove the sample form the instrument
- Insert the next sample
- Press <Go>
- Repeat until all samples have been read
Calculation the Concentration of your Sample[edit]
- Qubuit fluorometer gives values in either ug/ml or ng/ml
- Concentration of your sample = QF value x (200/x)
*where QF = the value given by the Qubit fluorometer x = the number of microliters of sample you added to the assay tube
- This equation generates a result with the same units as the value given by the Qubit fluorometer