HUES: Difference between revisions
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=='''HUES Cell Collection'''== | =='''HUES Cell Collection'''== | ||
==HUES homepage== | |||
*HUES homepage[http://www.mcb.harvard.edu/melton/HUES/index.html] | |||
==Protocol== | ==Protocol== | ||
*Protocol [[Media:hues.pdf]] | *Protocol [[Media:hues.pdf]] | ||
==Storage== | ==Storage== | ||
*Location: | *Location:CERC LN Tank | ||
==HUES Cell Log== | ==HUES Cell Log== | ||
*1 x HUES-3 p22 | *1 x HUES-3 p22 | ||
Line 13: | Line 16: | ||
*1 x HUES-66 p12 | *1 x HUES-66 p12 | ||
*2 x PMEFi | *2 x PMEFi | ||
==Materials and Reagents== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Materials / Catalogue no. ''' | |||
| align="center" style="background:#f0f0f0;"|'''Provider''' | |||
| align="center" style="background:#f0f0f0;"|'''CAT#''' | |||
|- | |||
| |||| | |||
|- | |||
| KO‐DMEM || Invitrogen Gibco || CAT#10829 | |||
|- | |||
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|- | |||
| DMEM ||Invitrogen Gibco ||CAT#11965‐092 | |||
|- | |||
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|- | |||
| FBS ||Valley Biomedical, Inc||CAT#BS‐3033 | |||
|- | |||
| |||| | |||
|- | |||
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|- | |||
| KO‐Serum Replacenment ||Invitrogen Gibco ||CAT#10828 | |||
|- | |||
| |||| | |||
|- | |||
| Plasmanate ||Bayer||CAT#NDC 0026‐0613‐20 | |||
|- | |||
| |||| | |||
|- | |||
| bFGF ||Invitrogen Gibco ||CAT#13256‐029 | |||
|- | |||
| |||| | |||
|- | |||
| hLIF ||Chemicon International||CAT#LIF1010 | |||
|- | |||
| |||| | |||
|- | |||
| 2‐beta‐mercaptoethanol||Gibco||CAT#21985‐023 | |||
|- | |||
| |||| | |||
|- | |||
| Non‐essential amino acids (NEAA) ||Invitrogen Gibco ||CAT#11140050 | |||
|- | |||
| (10mM or 100x)|||| | |||
|- | |||
| |||| | |||
|- | |||
| Pen/Strep ||Invitrogen Gibco ||CAT#15070‐063 | |||
|- | |||
| (5000U/ml Penicillin G sodium and|||| | |||
|- | |||
| 5000ug/ml Streptomycin sulfate in 0.85% saline) |||| | |||
|- | |||
| |||| | |||
|- | |||
| Glutamax‐I ||Invitrogen Gibco ||CAT#35050‐061 | |||
|- | |||
| (200mM or 100x) |||| | |||
|- | |||
| |||| | |||
|- | |||
| Mitomycin C||Sigma ||CAT#M‐0503 | |||
|- | |||
| |||| | |||
|- | |||
| 0.05% Trypsin/EDTA||Invitrogen Gibco ||CAT#25300‐054 | |||
|- | |||
| |||| | |||
|- | |||
| Dimethyl sulphoxide (DMSO)||Sigma ||CAT#D2650 | |||
|- | |||
| |||| | |||
|- | |||
| Gelatin ||Sigma ||CAT#G1890 | |||
|- | |||
| |||| | |||
|- | |||
| Tissue Culture Treated Plates (6 well)||Coring||CAT#3506 | |||
|- | |||
| |||| | |||
|- | |||
| Hygromycin resistant, pre-treated MEFs ||Specialty Media||CAT#PMEF‐H | |||
|- | |||
| |||| | |||
|- | |||
| Neomycin resistant, pre-treated MEFs||Specialty Media||CAT#PMEF‐N | |||
|- | |||
| | |||
|} | |||
==Contact Information== | ==Contact Information== | ||
==HUES Cell Facility Contact Information== | ==HUES Cell Facility Contact Information== |
Latest revision as of 23:49, 20 May 2011
HUES Cell Collection[edit]
HUES homepage[edit]
- HUES homepage[1]
Protocol[edit]
- Protocol Media:hues.pdf
Storage[edit]
- Location:CERC LN Tank
HUES Cell Log[edit]
- 1 x HUES-3 p22
- 1 x HUES-8 p19
- 1 x HUES-53 p11
- 1 x HUES-62 p10
- 1 x HUES-64 p12
- 1 x HUES-66 p12
- 2 x PMEFi
Materials and Reagents[edit]
Materials / Catalogue no. | Provider | CAT# |
KO‐DMEM | Invitrogen Gibco | CAT#10829 |
DMEM | Invitrogen Gibco | CAT#11965‐092 |
FBS | Valley Biomedical, Inc | CAT#BS‐3033 |
KO‐Serum Replacenment | Invitrogen Gibco | CAT#10828 |
Plasmanate | Bayer | CAT#NDC 0026‐0613‐20 |
bFGF | Invitrogen Gibco | CAT#13256‐029 |
hLIF | Chemicon International | CAT#LIF1010 |
2‐beta‐mercaptoethanol | Gibco | CAT#21985‐023 |
Non‐essential amino acids (NEAA) | Invitrogen Gibco | CAT#11140050 |
(10mM or 100x) | ||
Pen/Strep | Invitrogen Gibco | CAT#15070‐063 |
(5000U/ml Penicillin G sodium and | ||
5000ug/ml Streptomycin sulfate in 0.85% saline) | ||
Glutamax‐I | Invitrogen Gibco | CAT#35050‐061 |
(200mM or 100x) | ||
Mitomycin C | Sigma | CAT#M‐0503 |
0.05% Trypsin/EDTA | Invitrogen Gibco | CAT#25300‐054 |
Dimethyl sulphoxide (DMSO) | Sigma | CAT#D2650 |
Gelatin | Sigma | CAT#G1890 |
Tissue Culture Treated Plates (6 well) | Coring | CAT#3506 |
Hygromycin resistant, pre-treated MEFs | Specialty Media | CAT#PMEF‐H |
Neomycin resistant, pre-treated MEFs | Specialty Media | CAT#PMEF‐N |
Contact Information[edit]
HUES Cell Facility Contact Information[edit]
- HUES Facility / Melton Laboratory / HHMI
- Harvard University
- Cambridge, MA 02138
- Fax: 617.384.9463
- Please send all HUES cell correspondence to hues@mcb.harvard.edu.
Harvard's Office of Technology Development Contact Information[edit]
- Harvard's Office of Technology Development
- 1350 Massachusetts Avenue
- Holyoke Center 727
- Cambridge, MA 02138
- Fax: 617.495.9568
- Please send all correspondence concerning the MTA to Material Transfers: materialtransfer@harvard.edu.