Kun:LabNotes/ExonomeSeq/2008-1-15: Difference between revisions
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(New page: ==Exp.1 PCR on the capturing reaction started on 01/13/08== Template: 20ul 10X JumpStart buffer: 18ul 10mM dNTP 4ul 100uM AmpFV6.2Sol 0....) |
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94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold. | 94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold. | ||
<b> Amplification curves didn't came up at all. I added more enzyme and repeat the thermalcycling, still no signal. Didn't check with gel electrophoresis. </b> |
Latest revision as of 00:55, 18 January 2008
Exp.1 PCR on the capturing reaction started on 01/13/08[edit]
Template: 20ul 10X JumpStart buffer: 18ul 10mM dNTP 4ul 100uM AmpFV6.2Sol 0.8ul 100uM AmpRV6.2Sol 0.8ul 50X SYBG I 0.8ul JumpStart Taq 4ul H2O 152ul
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.
Amplification curves didn't came up at all. I added more enzyme and repeat the thermalcycling, still no signal. Didn't check with gel electrophoresis.