Kun:LabNotes/ExonomeSeq/2008-1-15: Difference between revisions

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(New page: ==Exp.1 PCR on the capturing reaction started on 01/13/08== Template: 20ul 10X JumpStart buffer: 18ul 10mM dNTP 4ul 100uM AmpFV6.2Sol 0....)
 
 
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94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.
<b> Amplification curves didn't came up at all. I added more enzyme and repeat the thermalcycling, still no signal. Didn't check with gel electrophoresis. </b>

Latest revision as of 00:55, 18 January 2008

Exp.1 PCR on the capturing reaction started on 01/13/08[edit]

    Template:              20ul
    10X JumpStart buffer:  18ul
    10mM dNTP               4ul
    100uM AmpFV6.2Sol     0.8ul
    100uM AmpRV6.2Sol     0.8ul
    50X SYBG I            0.8ul
    JumpStart Taq           4ul
    H2O                   152ul

94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.

Amplification curves didn't came up at all. I added more enzyme and repeat the thermalcycling, still no signal. Didn't check with gel electrophoresis.