Kun:LabNotes/ExonomeSeq/2008-1-16: Difference between revisions
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(New page: ==Exp.1 Perform another round of exon capture experiment== NA10835(347ng/ul) 3ul 3ul E55kv1 probes 180nM (01/12/08,purified) 10ul 275nM 7ul ...) |
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10X Ampligase buffer 1.5ul 1ul | 10X Ampligase buffer 1.5ul 1ul | ||
95C 10min -> 60C 40h -> add 2.5ul NSL mix (Stoffel | 95C 10min -> 60C 40h -> add 2.5ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 50uM) -> 60C 1h - -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold. |
Latest revision as of 18:24, 18 January 2008
Exp.1 Perform another round of exon capture experiment[edit]
NA10835(347ng/ul) 3ul 3ul E55kv1 probes 180nM (01/12/08,purified) 10ul 275nM 7ul 10X Ampligase buffer 1.5ul 1ul
95C 10min -> 60C 40h -> add 2.5ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 50uM) -> 60C 1h - -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.