Dinh:LAB/Diet Methylation: Difference between revisions

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* [[Dinh:Protocols/Probes_Prep_Oct6| Oct 6 2009 Probes Preparation]], CpG30K, 96rxns x 3, Nanodrop: 22.2 ug (last plate: Master Cycler), 55.5 ug (RT-BioRad), 51.5 ug (BioRad). End Point Gel Quantification: 13ng/ul x 46ul
* [[Dinh:Protocols/Probes_Prep_Oct6| Oct 6 2009 Probes Preparation]], CpG30K, 96rxns x 3, Nanodrop: 22.2 ug (last plate: Master Cycler), 55.5 ug (RT-BioRad), 51.5 ug (BioRad). End Point Gel Quantification: 13ng/ul x 46ul
* [[Dinh:Protocols/Probes_Prep_Oct27| Oct 27 2009 Probes Preparation]], CpG30K, 96rxns, Nanodrop: 67.12 ng/ul (32.2 ug). End Point Gel Quantification: 12ng/ul x 20ul
* [[Dinh:Protocols/Probes_Prep_Oct27| Oct 27 2009 Probes Preparation]], CpG30K, 96rxns, Nanodrop: 67.12 ng/ul (32.2 ug). End Point Gel Quantification: 12ng/ul x 20ul
* [[Dinh:Protocols/Probes_Prep_Nov2| Nov 2 2009 Probes Preparation]], CpG30K, 96rxns, Nanodrop: 115ng/ul (57.5 ug). End Point Gel Quantification: 7ng/ul x 11ul
* [[Dinh:Protocols/Probes_Prep_Nov6| Nov 6 2009 Probes Preparation]], CpG30K, 96rxns, Nanodrop: 120ng/ul (60.0 ug). End Point Gel Quantification: 12ng/ul x 22ul
*[[Dinh:Protocols/Probes_Prep_Nov17| Nov 17 2009 Probes Preparation]], CpG30K, 3 plates of 96rxns, Taq2xMM from NEB, Nanodrop: 100ng/ul (72ug).
*[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems.
=Capturing=
* [[Dinh:Protocols/Capturing_Nov13| Nov 13 2009 Capturing - Practice]], CpG30K, Positive control: Jurkat, Negative control: RNAse free-ddH20

Latest revision as of 22:08, 24 November 2009

Probes Preparation[edit]

Capturing[edit]