Noi:Probe preparation data/Probes Prep: Difference between revisions
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>Noi (Created page with '=Summary of DMR220k probe set production= {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''Probe set''' | align="center" style="background:#f0f0f0;"|'''No…') |
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| align="center" style="background:#f0f0f0;"|'''for DNA capture''' | | align="center" style="background:#f0f0f0;"|'''for DNA capture''' | ||
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| S1||96 rxn||2010_10_24||Kapa||26. | | S1||96 rxn||2010_10_24||Kapa||26.20||250.00||6.50||diluted to 2ng/ul vol 100ul | ||
|- | |- | ||
| S2||96 rxn||2010_10_24||Kapa||30. | | S2||96 rxn||2010_10_24||Kapa||30.10||250.00||7.50||diluted to 2ng/ul vol 100ul | ||
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| S3||96 rxn||2010_10_24||Kapa||23. | | S3||96 rxn||2010_10_24||Kapa||23.20||250.00||5.80|| | ||
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| S4||96 rxn||2010_10_21||Kapa||24. | | S4||96 rxn||2010_10_21||Kapa||24.80||200.00||4.90|| | ||
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| S5||96 rxn||2010_10_21||Kapa||26. | | S5||96 rxn||2010_10_21||Kapa||26.70||200.00||5.30|| | ||
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| S6||96 rxn||2010_10_22||Kapa||27. | | S6||96 rxn||2010_10_22||Kapa||27.70||200.00||5.50|| | ||
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| S7||96 rxn||2010_10_22||Kapa||32||200||6. | | S7||96 rxn||2010_10_22||Kapa||32.00||200.00||6.40|| | ||
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| 1st prep||||||||||||||Total (ug) | | 1st prep||||||||||||||Total (ug) | ||
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| S6||96 rxn||2010_10_01||EconoTaq||24. | | S6||96 rxn||2010_10_01||EconoTaq||24.60||102.00||2.50||8.00 | ||
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| S7||96 rxn||2010_09_27||Kapa||36. | | S7||96 rxn||2010_09_27||Kapa||36.90||200.00||7.30||13.70 | ||
|}<br> | |||
{| {{table}} border = 1 | |||
| align="center" style="background:#f0f0f0;"|'''Probe set''' | |||
| align="center" style="background:#f0f0f0;"|'''Amplified amplicon yields (ug)''' | |||
| align="center" style="background:#f0f0f0;"|'''ss-amplicon yields (ug)''' | |||
| align="center" style="background:#f0f0f0;"|'''USER/DpnII digested DNA yields (ug)''' | |||
| align="center" style="background:#f0f0f0;"|'''% digestion, %recovery''' | |||
| align="center" style="background:#f0f0f0;"|'''PAGE size selection yields (ug)''' | |||
|- | |||
| S1||86.6||22.2||13||92%, 54% ||6.5 | |||
|- | |||
| S2 ||97||26.5||15.6||92%, 52% ||7.5 | |||
|- | |||
| S3 ||97.2||24.4||13.8||94%, 44% ||5.8 | |||
|- | |||
| S4 ||95.5||19.5||10.7||93%, 49% ||4.9 | |||
|- | |||
| S5 ||95.8||18.2||9.6||92%, 60% ||5.3 | |||
|- | |||
| S6||95.8||23.7||12.2||>90%, 51% ||5.5 | |||
|- | |||
| EconoTaq ||58.5||8.7||5.1||||2.5 | |||
|- | |||
| S7 ||88.6||23.5||12.4||93%, 55% ||6.4 | |||
|- | |||
| ||95.4||21.2||11.8||||7.3 | |||
|} | |} |
Latest revision as of 01:06, 9 December 2010
Summary of DMR220k probe set production[edit]
Probe set | No. of rxn | Date of production | Enzyme | Conc. (ng/ul) | Vol. (ul) | Yields (ug) | for DNA capture |
S1 | 96 rxn | 2010_10_24 | Kapa | 26.20 | 250.00 | 6.50 | diluted to 2ng/ul vol 100ul |
S2 | 96 rxn | 2010_10_24 | Kapa | 30.10 | 250.00 | 7.50 | diluted to 2ng/ul vol 100ul |
S3 | 96 rxn | 2010_10_24 | Kapa | 23.20 | 250.00 | 5.80 | |
S4 | 96 rxn | 2010_10_21 | Kapa | 24.80 | 200.00 | 4.90 | |
S5 | 96 rxn | 2010_10_21 | Kapa | 26.70 | 200.00 | 5.30 | |
S6 | 96 rxn | 2010_10_22 | Kapa | 27.70 | 200.00 | 5.50 | |
S7 | 96 rxn | 2010_10_22 | Kapa | 32.00 | 200.00 | 6.40 | |
1st prep | Total (ug) | ||||||
S6 | 96 rxn | 2010_10_01 | EconoTaq | 24.60 | 102.00 | 2.50 | 8.00 |
S7 | 96 rxn | 2010_09_27 | Kapa | 36.90 | 200.00 | 7.30 | 13.70 |
Probe set | Amplified amplicon yields (ug) | ss-amplicon yields (ug) | USER/DpnII digested DNA yields (ug) | % digestion, %recovery | PAGE size selection yields (ug) |
S1 | 86.6 | 22.2 | 13 | 92%, 54% | 6.5 |
S2 | 97 | 26.5 | 15.6 | 92%, 52% | 7.5 |
S3 | 97.2 | 24.4 | 13.8 | 94%, 44% | 5.8 |
S4 | 95.5 | 19.5 | 10.7 | 93%, 49% | 4.9 |
S5 | 95.8 | 18.2 | 9.6 | 92%, 60% | 5.3 |
S6 | 95.8 | 23.7 | 12.2 | >90%, 51% | 5.5 |
EconoTaq | 58.5 | 8.7 | 5.1 | 2.5 | |
S7 | 88.6 | 23.5 | 12.4 | 93%, 55% | 6.4 |
95.4 | 21.2 | 11.8 | 7.3 |