Rui:LabNotes/SingleCell/2011-1-20: Difference between revisions
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>RuiLiu (Created page with '1. Measure the concentration of the RNA samples *Hues 54 414.50ng/ul 2.06 2.01 *Hues6-ES 641.13ng/ul 2.12 2.11 *Hues6-EB 561.99ng/ul 2.11 2.11 2. Examine the int…') |
>RuiLiu |
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1. Measure the concentration of the RNA samples | =1. Measure the concentration of the RNA samples= | ||
-------------------------------------------------------------- | |||
RNA ID [ng/ul] 260/280 260/230 | |||
-------------------------------------------------------------- | |||
Hues54 414.50 2.06 2.01 | |||
Hues6-ES 641.13 2.12 2.11 | |||
Hues6-EB 561.99 2.11 2.11 | |||
-------------------------------------------------------------- | |||
2. Examine the integrity of the RNA samples | =2. Examine the integrity of the RNA samples= | ||
* | * 25ml agarose gel + 2ul SYBR safe | ||
[[File:ZhangLab_2 2011-01-20 11hr 50min.jpg]] | |||
3. Dilute RNA samples in PCR 8-tube strip (-80C) | =3. Dilute RNA samples in PCR 8-tube strip (-80C)= | ||
*1) 1:10 | *1) 1:10 | ||
*2) 1:10 | *2) 1:10 |
Latest revision as of 18:24, 21 January 2011
1. Measure the concentration of the RNA samples[edit]
-------------------------------------------------------------- RNA ID [ng/ul] 260/280 260/230 -------------------------------------------------------------- Hues54 414.50 2.06 2.01 Hues6-ES 641.13 2.12 2.11 Hues6-EB 561.99 2.11 2.11 --------------------------------------------------------------
2. Examine the integrity of the RNA samples[edit]
- 25ml agarose gel + 2ul SYBR safe
File:ZhangLab 2 2011-01-20 11hr 50min.jpg
3. Dilute RNA samples in PCR 8-tube strip (-80C)[edit]
- 1) 1:10
- 2) 1:10
- 3) 1:4/1:6/1:5.6 --> 1ng/ul
- 4) 1:5 --> 0.2ng/ul
- 5) 1:10 --> 0.02ng/ul