Rui:LabNotes/SingleCell/2011-1-20: Difference between revisions

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=1. Measure the concentration of the RNA samples=
=1. Measure the concentration of the RNA samples=
  --------------------------------------------------------------
  --------------------------------------------------------------
  RNA ID [ng/ul] 260/280 260/230
  RNA ID         [ng/ul]       260/280         260/230
  --------------------------------------------------------------  
  --------------------------------------------------------------  
  Hues54 414.50         2.06         2.01
  Hues54         414.50         2.06         2.01
  Hues6-ES 641.13         2.12         2.11
  Hues6-ES       641.13         2.12         2.11
  Hues6-EB 561.99         2.11         2.11
  Hues6-EB       561.99         2.11         2.11
  --------------------------------------------------------------
  --------------------------------------------------------------


=2. Examine the integrity of the RNA samples=
=2. Examine the integrity of the RNA samples=
* 100ml agarose gel + 8ul SYBR safe
* 25ml agarose gel + 2ul SYBR safe
[[File:ZhangLab_2 2011-01-20 11hr 50min.jpg]]


=3. Dilute RNA samples in PCR 8-tube strip (-80C)=
=3. Dilute RNA samples in PCR 8-tube strip (-80C)=
1) 1:10
*1) 1:10
2) 1:10
*2) 1:10
3) 1:4/1:6/1:5.6 --> 1ng/ul
*3) 1:4/1:6/1:5.6 --> 1ng/ul
4) 1:5 --> 0.2ng/ul
*4) 1:5 --> 0.2ng/ul
5) 1:10 --> 0.02ng/ul
*5) 1:10 --> 0.02ng/ul

Latest revision as of 18:24, 21 January 2011

1. Measure the concentration of the RNA samples[edit]

--------------------------------------------------------------
RNA ID          [ng/ul]        260/280         260/230
-------------------------------------------------------------- 
Hues54          414.50	        2.06	        2.01
Hues6-ES        641.13	        2.12	        2.11
Hues6-EB        561.99	        2.11	        2.11
--------------------------------------------------------------

2. Examine the integrity of the RNA samples[edit]

  • 25ml agarose gel + 2ul SYBR safe

File:ZhangLab 2 2011-01-20 11hr 50min.jpg

3. Dilute RNA samples in PCR 8-tube strip (-80C)[edit]

  • 1) 1:10
  • 2) 1:10
  • 3) 1:4/1:6/1:5.6 --> 1ng/ul
  • 4) 1:5 --> 0.2ng/ul
  • 5) 1:10 --> 0.02ng/ul