Rui:LabNotes/SingleCell/2011-5-23: Difference between revisions

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==Library construct by Nextera==
===Procedure===
* 50ng samples: sperm RNA, ES RNA, sperm lysate, ES lysates
* 5ul tagmentation: 0.25ul transposon, 1ul LMW buffer
* 50ul Kapa FAST PCR: barcode adapter 2 (ID9-12), 10 cycles
* Beads purification, elute in 20ul


===QPCR curve===
[[File:Nextera_5.23.11.jpg]]
===TBE PAGE===
[[File:50ng Nextera PCR_5.23.11.jpg]]
==Library construct by ligation with Y adaptor==
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''>Digestion'''
| align="center" style="background:#f0f0f0;"|'''ng/ul'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''260/230'''
|-
| sperm RNA||39.12||1.9||2.77
|-
| ES RNA||51.57||1.92||1.92
|-
| sperm lysis||16.27||1.73||1.42
|-
| ES lysis||45.64||1.93||1.99
|-
| ||||||
|-
| ||||||
|-
| >Ligation||ng/ul ||260/280 ||260/230
|-
| sperm RNA||35.03||2.05||2.19
|-
| ES RNA||45.66||1.92||1.99
|-
| sperm lysis||13.99||2.04||1.7
|-
| ES lysis||39.23||2.06||2.12
|-
| ||||||
|-
| ||||||
|-
| >PCR||ng/ul ||260/280 ||260/230
|-
| sperm RNA ID5||21.29||1.73||1.69
|-
| ES RNA ID6||21.31||1.72||1.7
|-
| sperm lysis ID7||19.24||1.61||1.14
|-
| ES lysis ID8||20.03||1.74||1.1
|-
|
|}
==Cloning of PCR product==

Latest revision as of 21:08, 17 June 2011