Arichard:Samples/MDA microarray samples: Difference between revisions
Jump to navigation
Jump to search
>Andrew No edit summary |
>Andrew |
||
(2 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
==MDA microarray== | ==MDA microarray== | ||
===September 13=== | |||
* All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum). | * All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum). | ||
** 2401 CTX+ needs no dilution. | ** 2401 CTX+ needs no dilution. | ||
Line 10: | Line 10: | ||
** Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul). | ** Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul). | ||
** Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume. | ** Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume. | ||
* Eukaryotic samples are stored at 4deg C. Prokaryotic samples are stored at -20deg C as glycerol stocks. | |||
===October 10=== | |||
* Below is the current list of all the samples and required dilutions. | |||
{| border="1" | |||
|+ Samples and dilutions | |||
| Skn || 1:1 | |||
|- | |||
| Sea || 1:1 | |||
|- | |||
| CtlCtx+ || 1:10 | |||
|- | |||
| CtlCtx- || 1:10 | |||
|- | |||
| AlzCtx+ || 1:1 | |||
|- | |||
|AlzCtx- || 1:10 | |||
|- | |||
|AlzCbl+ || 1:20 | |||
|- | |||
|AlzCbl- || 1:10 | |||
|- | |||
| DowCtx+ || 1:10 | |||
|- | |||
| DowCtx || 1:20 | |||
|} | |||
===09/25/2013=== | |||
Four new samples from Chun lab received last week: | |||
* Non-diseased, neuronal (1568 +) | |||
* Non-diseased, non-neuronal (1568 -) | |||
* Alzheimer's, neuronal (25-00 +) | |||
* Alzheimer's, non-neuronal (25-00 -) | |||
According to Gwen: Fixed in ethanol, suspended in PBS. |
Latest revision as of 22:49, 25 September 2013
MDA microarray[edit]
September 13[edit]
- All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum).
- 2401 CTX+ needs no dilution.
- 2401 CBL+ needs 1:10 dilution.
- Jeff didn't say what dilutions are needed for 2401 CTX- and 2401 CBL-.
- Other samples are as follows
- Two types of sea bacteria: Synechococcus and prochlorococcus. These samples are already at the right concentrations, 10-20 parts/ul.
- Skin bacteria sample from microbiome project. Heterogeneous. I need to ask Jeff about these dilutions.
- Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul).
- Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume.
- Eukaryotic samples are stored at 4deg C. Prokaryotic samples are stored at -20deg C as glycerol stocks.
October 10[edit]
- Below is the current list of all the samples and required dilutions.
Skn | 1:1 |
Sea | 1:1 |
CtlCtx+ | 1:10 |
CtlCtx- | 1:10 |
AlzCtx+ | 1:1 |
AlzCtx- | 1:10 |
AlzCbl+ | 1:20 |
AlzCbl- | 1:10 |
DowCtx+ | 1:10 |
DowCtx | 1:20 |
09/25/2013[edit]
Four new samples from Chun lab received last week:
- Non-diseased, neuronal (1568 +)
- Non-diseased, non-neuronal (1568 -)
- Alzheimer's, neuronal (25-00 +)
- Alzheimer's, non-neuronal (25-00 -)
According to Gwen: Fixed in ethanol, suspended in PBS.