Hosuk:LabNotesMDA/2013-2-5: Difference between revisions

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>Hosuki78
(Created page with "====qPCR run==== *Run qPCR with the sample from 1/28, 1/29 and 2/4 using mouse and human primer *The number of tubes **1/28: 16ea (#9~#24) **1/29: 21ea (#1~#21) **2/4: 23ea (...")
 
>Hosuki78
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====qPCR run====
*(will be added)
 
*Run qPCR with the sample from 1/28, 1/29 and 2/4 using mouse and human primer
*The number of tubes
**1/28: 16ea (#9~#24)
**1/29: 21ea (#1~#21)
**2/4: 23ea (#1~#24)
*Preparation
**1/28 samples: 8 tubes by taking 0.5uL from two tubes and merging into one sample
**1/28 samples: 8 tubes by taking 0.5uL from two tubes and merging into one sample
*PCR master mix
**KAPA fast
***KAPA fast 15 uL
***10 uM primer 1 .6 uL
***10 uM primer 2 .6 uL
***DNA from library 2 uL
***water 11.4 uL
***total 30 uL
PCR reaction
**95C 10sec
**58C 20sec
**72C 45sec
**40cycle
 
====Result====
 
*30 C for 6 hour reaction

Latest revision as of 17:40, 6 February 2013

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