Blue:RNA-Seq Experiments:03212013: Difference between revisions

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Purpose: Test RNAseIII single cell totoRNAseq protocol - Same as for 03192013 except with a longer RNAseIII incubation time (increased from 10min to 20min)


Result: Only minor downward shift in library fragment size - still too many high molecular weight fragments and poor amplification after DSN treatment




[[File:03212013.pdf]]
[[File:03212013.pdf]]

Latest revision as of 22:11, 25 March 2013

Purpose: Test RNAseIII single cell totoRNAseq protocol - Same as for 03192013 except with a longer RNAseIII incubation time (increased from 10min to 20min)


Result: Only minor downward shift in library fragment size - still too many high molecular weight fragments and poor amplification after DSN treatment


File:03212013.pdf